Single cell transcriptome atlas of the Drosophila larval brain
- Brunet Avalos, Clarisse Department of Biology, University of Fribourg, Fribourg, Switzerland
- Maier, G. Larisa Department of Biology, University of Fribourg, Fribourg, Switzerland
- Bruggmann, Rémy Interfaculty Bioinformatics Unit, University of Bern, Bern, Switzerland
- Sprecher, Simon G. Department of Biology, University of Fribourg, Fribourg, Switzerland
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20.11.2019
Published in:
- eLife. - 2019, vol. 8, p. e50354
English
Cell diversity of the brain and how it is affected by starvation, remains largely unknown. Here, we introduce a single cell transcriptome atlas of the entire Drosophila first instar larval brain. We first assigned cell-type identity based on known marker genes, distinguishing five major groups: neural progenitors, differentiated neurons, glia, undifferentiated neurons and non-neural cells. All major classes were further subdivided into multiple subtypes, revealing biological features of various cell-types. We further assessed transcriptional changes in response to starvation at the single- cell level. While after starvation the composition of the brain remains unaffected, transcriptional profile of several cell clusters changed. Intriguingly, different cell-types show very distinct responses to starvation, suggesting the presence of cell-specific programs for nutrition availability. Establishing a single-cell transcriptome atlas of the larval brain provides a powerful tool to explore cell diversity and assess genetic profiles from developmental, functional and behavioral perspectives.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 327947
- DOI 10.7554/eLife.50354
- Persistent URL
- https://folia.unifr.ch/unifr/documents/308181
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