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Local accumulation of diacylglycerol alters membrane properties nonlinearly due to its transbilayer activity

  • Campomanes, Pablo Department of Biology, University of Fribourg, Switzerland
  • Zoni, Valeria Department of Biology, University of Fribourg, Switzerland
  • Vanni, Stefano Department of Biology, University of Fribourg, Switzerland
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  • Communications Chemistry. - 2019, vol. 2, no. 1, p. 72
English Diacylglycerols (DAGs) are bioactive lipids that are ubiquitously present at low concentrations in cellular membranes. Upon the activation of lipid remodeling enzymes such as phospholipase C and phosphatidic acid phosphatase, DAG concentration increases, leading to a disruption of the lamellar phase of lipid membranes. To investigate the structural origin of these phenomena, here we develop a coarse-grained model for DAGs that is able to correctly reproduce its physicochemical properties, including interfacial tension and flip-flop rate. We find that even at low concentrations a nonnegligible percentage of DAG molecules occupies the interleaflet space. At high concentrations, DAG molecules undergo a phase- separation process from lamellar lipids, segregating in DAG-only blisters and effectively reducing the DAG surface pool available to peripheral enzymes. Our results allow for a better understanding of the role of DAGs in cellular membranes and provide a new tool for the quantitative estimation of low-abundance lipids on membrane properties.
Faculté des sciences et de médecine
Département de Biologie
  • English
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