Journal article

VAPYRIN Marks an endosomal trafficking compartment involved in arbuscular mycorrhizal symbiosis

  • Bapaume, Laure Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Laukamm, Sabine Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Darbon, Geoffrey Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Monney, Corinne Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Meyenhofer, Felix Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Feddermann, Nadja Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Chen, Min Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Reinhardt, Didier Department of Biology, University of Fribourg, Fribourg, Switzerland
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    2019
Published in:
  • Frontiers in Plant Science. - 2019, vol. 10
English Arbuscular mycorrhiza (AM) is a symbiosis between plants and AM fungi that requires the intracellular accommodation of the fungal partner in the host. For reciprocal nutrient exchange, AM fungi form intracellular arbuscules that are surrounded by the peri-arbuscular membrane. This membrane, together with the fungal plasma membrane, and the space in between, constitute the symbiotic interface, over which nutrients are exchanged. Intracellular establishment of AM fungi requires the VAPYRIN protein which is induced in colonized cells, and which localizes to numerous small mobile structures of unknown identity (Vapyrin-bodies). In order to characterize the identity and function of the Vapyrin-bodies we pursued a dual strategy. First, we co-expressed fluorescently tagged VAPYRIN with a range of subcellular marker proteins, and secondly, we employed biochemical tools to identify interacting partner proteins of VAPYRIN. As an important tool for the quantitative analysis of confocal microscopic data sets from co-expression of fluorescent proteins, we developed a semi-automated image analysis pipeline that allows for precise spatio-temporal quantification of protein co-localization and of the dynamics of organelle association from movies. Taken together, these experiments revealed that Vapyrin-bodies have an endosomal identity with trans-Golgi features, and that VAPYRIN interacts with a symbiotic R-SNARE of the VAMP721 family, that localizes to the same compartment.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307941
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