Journal article

Characterization of PAN-1, a carbapenem-hydrolyzing class b β-lactamase from the environmental gram-negative Pseudobacteriovorax antillogorgiicola

  • Kieffer, Nicolas Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Switzerland
  • Poirel, Laurent Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Department of Medicine, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
  • Fournier, Claudine Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Switzerland
  • Haltli, Brad Department of Biomedical Sciences, Atlantic Veterinary College, Charlottetown, Canada
  • Kerr, Russel Department of Biomedical Sciences, Atlantic Veterinary College, Charlottetown, Canada
  • Nordmann, Patrice Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Department of Medicine, INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Department of Medicine, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - Department of Medicine, Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland
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    2019
Published in:
  • Frontiers in Microbiology. - 2019, vol. 10
English The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species Pseudobacteriovorax antillogorgiicola. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM- 1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307933
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