Annexin A7 is required for ESCRT III-mediated plasma membrane repair
- Sønder, Stine Lauritzen Unit for Cell Death and Metabolism, Danish Cancer Society Research Center, Copenhagen, Denmark
- Boye, Theresa Louise Unit for Cell Death and Metabolism, Danish Cancer Society Research Center, Copenhagen, Denmark
- Tölle, Regine Department of Dermatology, Medical Center, University of Freiburg, Germany - Department of Biology, University of Fribourg, Switzerland
- Dengjel, Jörn Department of Dermatology, Medical Center, University of Freiburg, Germany - Department of Biology, University of Fribourg, Switzerland
- Maeda, Kenji Unit for Cell Death and Metabolism, Danish Cancer Society Research Center, Copenhagen, Denmark
- Jäättelä, Marja Unit for Cell Death and Metabolism, Danish Cancer Society Research Center, Copenhagen, Denmark - Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark
- Simonsen, Adam Cohen Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense M, Denmark
- Jaiswal, Jyoti K. Children’s National Health System, Center for Genetic Medicine Research, George Washington University School of Medicine and Health Sciences, Washington, USA - Department of Genomics and Precision Medicine, George Washington University School of Medicine and Health Sciences, Washington, USA
- Nylandsted, Jesper Unit for Cell Death and Metabolism, Danish Cancer Society Research Center, Copenhagen, Denmark - Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark
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30.04.2019
Published in:
- Scientific Reports. - 2019, vol. 9, no. 1, p. 6726
English
The plasma membrane of eukaryotic cells forms the essential barrier to the extracellular environment, and thus plasma membrane disruptions pose a fatal threat to cells. Here, using invasive breast cancer cells we show that the Ca2+ - and phospholipid-binding protein annexin A7 is part of the plasma membrane repair response by enabling assembly of the endosomal sorting complex required for transport (ESCRT) III. Following injury to the plasma membrane and Ca2+ flux into the cytoplasm, annexin A7 forms a complex with apoptosis linked gene-2 (ALG-2) to facilitate proper recruitment and binding of ALG-2 and ALG-2-interacting protein X (ALIX) to the damaged membrane. ALG-2 and ALIX assemble the ESCRT III complex, which helps excise and shed the damaged portion of the plasma membrane during wound healing. Our results reveal a novel function of annexin A7 – enabling plasma membrane repair by regulating ESCRT III-mediated shedding of injured plasma membrane.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 324628
- DOI 10.1038/s41598-019-43143-4
- Persistent URL
- https://folia.unifr.ch/unifr/documents/307815
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