Journal article
+ 1 other files
Hemozoin-catalyzed precipitation polymerization as an assay for malaria diagnosis
-
Rifaie-Graham, Omar
Adolphe Merkle Institute, University of Fribourg, Switzerland
-
Pollard, Jonas
Adolphe Merkle Institute, University of Fribourg, Switzerland
-
Raccio, Samuel
Adolphe Merkle Institute, University of Fribourg, Switzerland
-
Balog, Sandor
Adolphe Merkle Institute, University of Fribourg, Switzerland
-
Rusch, Sebastian
Swiss Tropical and Public Health Institute, Basel, Switzerland - University of Basel, Basel, Switzerland
-
Hernández-Castañeda, María Andrea
Department of Medicine, University of Fribourg, Switzerland
-
Mantel, Pierre-Yves
Department of Medicine, University of Fribourg, Switzerland
-
Beck, Hans-Peter
Swiss Tropical and Public Health Institute, Basel, Switzerland - University of Basel, Basel, Switzerland
-
Bruns, Nico
Adolphe Merkle Institute, University of Fribourg, Switzerland
Show more…
Published in:
- Nature Communications. - 2019, vol. 10, no. 1, p. 1369
English
Methods to diagnose malaria are of paramount interest to eradicate the disease. Current methods have severe limitations, as they are either costly or not sensitive enough to detect low levels of parasitemia. Here we report an ultrasensitive, yet low- resource chemical assay for the detection and quantification of hemozoin, a biomarker of all Plasmodium species. Solubilized hemozoin catalyzes the atom transfer radical polymerization of N-isopropylacrylamide above the lower critical solution temperature of poly(N-isopropylacrylamide). The solution becomes turbid, which can be observed by naked eye and quantified by UV-visible spectroscopy. The rate of turbidity increase is proportional to the concentration of hemozoin, with a detection limit of 0.85 ng mL−1. Malaria parasites in human blood can be detected down to 10 infected red blood cells μL−1. The assay could potentially be applied as a point-of-care test. The signal-amplification of an analyte by biocatalytic precipitation polymerization represents a powerful approach in biosensing.
-
Faculty
- Faculté des sciences et de médecine
-
Department
- Département de Médecine, AMI - Chimie des polymères et matériaux
-
Language
-
-
Classification
-
Biological sciences
-
License
-
License undefined
-
Identifiers
-
-
Persistent URL
-
https://folia.unifr.ch/unifr/documents/307790
Other files
Statistics
Document views: 37
File downloads:
- man_hcp.pdf: 86
- man_hcp_sm.pdf: 86