Journal article

Prevalence of fosfomycin resistance among ESBL-producing Escherichia coli isolates in the community, Switzerland

  • Mueller, Linda Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA)University of Fribourg, Switzerland
  • Cimen, Cansu Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Infectious Diseases and Clinical Microbiology Clinic, Ardahan Public Hospital, Ardahan, Turkey
  • Poirel, Laurent  Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA)University of Fribourg, Switzerland - INSERM European Unit (IAME/LEA, France)University of Fribourg, Switzerland
  • Descombes, Marie-Christine Laboratoire Proxilis, Meyrin, Switzerland
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA)University of Fribourg, Switzerland - INSERM European Unit (IAME/LEA, France)University of Fribourg, Switzerland - University Hospital Center and University of Lausanne, Lausanne, Switzerland
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    01.05.2019
Published in:
  • European Journal of Clinical Microbiology & Infectious Diseases. - 2019, vol. 38, no. 5, p. 945–949
English Our aim was to evaluate the prevalence of fosfomycin-resistant strains among ESBL- producing Escherichia coli isolates recovered from community patients in Switzerland. A total of 1225 ESBL-producing E. coli isolates were collected between 2012 and 2013 from a private and community laboratory. Fosfomycin resistance was assessed by using the novel rapid fosfomycin/E. coli NP test and agar dilution method. Resistant isolates were further investigated for acquired resistance genes fosA1–7 by PCR and sequencing. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) were performed to evaluate the clonal relationship among fosA3-carrying isolates. Out of the 1225 ESBL-producing E. coli isolates analyzed in this study, 1208 were fosfomycin susceptible while 17 were fosfomycin resistant. No discrepancy was observed between the rapid fosfomycin/E. coli NP test and the agar dilution method taken as the gold standard. Five out of the 17 resistant isolates carried a fosA-like gene. No clonal relationship was observed among those isolates. Here, the prevalence of fosfomycin resistance among ESBL-producing E. coli isolates in the community is reported for the first time in Switzerland, being ca. 1.4%. Among the five isolates carrying a fosA gene, four encoded the FosA3 enzyme, being the most prevalent fosfomycin-resistant determinant. An excellent correlation was observed between minimum inhibitory concentration–based susceptibility categorization and results of the rapid fosfomycin/E. coli NP test, further indicating the excellent sensitivity and specificity of this recently developed rapid test whose results are obtained in less than 2 h.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307768
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