The potential of antagonistic moroccan Streptomyces isolates for the biological control of damping-off disease of pea (Pisum sativum L.) caused by Aphanomyces euteiches

Oubaha, Brahim; Nafis, Ahmed; Baz, Mohamed; Mauch, Felix; Barakate, Mustapha

doi:10.1111/jph.12775

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Journal article

The potential of antagonistic moroccan Streptomyces isolates for the biological control of damping-off disease of pea (Pisum sativum L.) caused by Aphanomyces euteiches

Oubaha, Brahim Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco - Laboratory of Interaction Plant‐Microorganisms, Department of Biology University of Fribourg, Switzerland
Nafis, Ahmed Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
Baz, Mohamed Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco
Mauch, Felix Laboratory of Interaction Plant‐Microorganisms, Department of Biology University of Fribourg Fribourg Switzerland
Barakate, Mustapha Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakech, Morocco - Laboratory of Interaction Plant‐Microorganisms, Department of Biology University of Fribourg, Switzerland - Agrobiosciences & Fertilizers Program, University Mohammed IV, Polytechnic (UM6P), Benguerir, Morocco

15.12.2018

Published in:

Journal of Phytopathology. - 2019, vol. 167, no. 2, p. 82–90

English Three hundred and fifty‐nine isolates of actinobacteria collected from different Moroccan soils were evaluated for their in vitro antimicrobial activity against the oomycete pathogen Aphanomyces euteiches, the causal agent of damping‐off of pea and other legumes. Eighty‐seven isolates (24%) had an inhibitory in vitro effect against A. euteiches. Fourteen bioactive isolates with the greatest inhibitory effect against A. euteiches and no inhibitory effect on plant beneficial rhizobia were tested for their ability to protect pea seeds and seedlings against the damping‐off disease using culture supernatants or spore suspensions as treatments. The two most protective isolates, OB21 and BA15, significantly reduced, compared to untreated control plants, damping‐off by 33% and 47%, respectively. The two bioactive isolates were classified as species of the genus Streptomyces based on 16S rDNA analysis and morphological and chemical characteristics.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language