T cells loaded with magnetic nanoparticles are retained in peripheral lymph nodes by the application of a magnetic field
- Sanz‑Ortega, Laura Department of Immunology and Oncology, Centro Nacional de Biotecnología, Madrid, Spain
- Rojas, José M. Department of Immunology and Oncology, Centro Nacional de Biotecnología, Madrid, Spain - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain
- Marcos, Ana Theodor Kocher Institute, University of Bern, Switzerland - Section of Medicine, Department of Oncology, Microbiology and Immunology, University of Fribourg, Switzerland
- Portilla, Yadileiny Department of Immunology and Oncology, Centro Nacional de Biotecnología, Madrid, Spain
- Stein, Jens V. Theodor Kocher Institute, University of Bern, Switzerland - Section of Medicine, Department of Oncology, Microbiology and Immunology, University of Fribourg, Switzerland
- Barber, Domingo F. Department of Immunology and Oncology, Centro Nacional de Biotecnología, Madrid, Spain
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22.01.2019
Published in:
- Journal of Nanobiotechnology. - 2019, vol. 17, no. 1, p. 14
English
T lymphocytes are highly dynamic elements of the immune system with a tightly regulated migration. T cell-based transfer therapies are promising therapeutic approaches which in vivo efficacy is often limited by the small proportion of administered cells that reaches the region of interest. Manipulating T cell localisation to improve specific targeting will increase the effectiveness of these therapies. Nanotechnology has been successfully used for localized release of drugs and biomolecules. In particular, magnetic nanoparticles (MNPs) loaded with biomolecules can be specifically targeted to a location by an external magnetic field (EMF). The present work studies whether MNP-loaded T cells could be targeted and retained in vitro and in vivo at a site of interest with an EMF.Results: T cells were unable to internalize the different MNPs used in this study, which remained in close association with the cell membrane. T cells loaded with an appropriate MNP concentration were attracted to an EMF and retained in an in vitro capillary flow-system. MNP-loaded T cells were also magnetically retained in the lymph nodes after adoptive transfer in in vivo models. This enhanced in vivo retention was in part due to the EMF application and to a reduced circulating cell speed within the organ. This combined use of MNPs and EMFs did not alter T cell viability or function.Conclusions: These studies reveal a promising approach to favour cell retention that could be implemented to improve cell- based therapy.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Médecine
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 324127
- DOI 10.1186/s12951-019-0440-z
- Persistent URL
- https://folia.unifr.ch/unifr/documents/307535
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