Interaction of the hydrogen sulfide system with the oxytocin system in the injured mouse heart
- Merz, Tamara Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany
- Lukaschewski, Britta Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany
- Wigger, Daniela Clinic for Psychsomatic Medicine and Psychotherapy, University Medical Center, Ulm, Germany
- Rupprecht, Aileen Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany
- Wepler, Martin Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany - Department of Anesthesiology, University Medical Center, Ulm, Germany
- Gröger, Michael Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany
- Hartmann, Clair Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany - Department of Anesthesiology, University Medical Center, Ulm, Germany
- Whiteman, Matthew University of Exeter Medical School, Exeter, UK
- Szabo, Csaba Chair of Pharmacology, Department of Oncology, Microbiology and Immunology, Faculty of Science and Medicine, University of Fribourg, Switzerland - Department of Anesthesiology, University of Texas Medical Branch, Galveston, USA
- Wang, Rui Department of Biology, Laurentian University, Sudbury, Canada
- Waller, Christiane Clinic for Psychsomatic Medicine and Psychotherapy, University Medical Center, Ulm, Germany - Department of Psychosomatic Medicine and Psychotherapy, Paracelsus Medical University, Nuremberg General Hospital, Nuremberg, Germany
- Radermacher, Peter Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany
- McCook, Oscar Institute of Anesthesiological Pathophysiology and Process Engineering, University Medical School, Ulm, Germany
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19.10.2018
Published in:
- Intensive Care Medicine Experimental. - 2018, vol. 6, no. 1, p. 41
English
Both the hydrogen sulfide/cystathionine-γ-lyase (H2S/CSE) and oxytocin/oxytocin receptor (OT/OTR) systems have been reported to be cardioprotective. H2S can stimulate OT release, thereby affecting blood volume and pressure regulation. Systemic hyper-inflammation after blunt chest trauma is enhanced in cigarette smoke (CS)-exposed CSE−/− mice compared to wildtype (WT). CS increases myometrial OTR expression, but to this point, no data are available on the effects CS exposure on the cardiac OT/OTR system. Since a contusion of the thorax (Txt) can cause myocardial injury, the aim of this post hoc study was to investigate the effects of CSE−/− and exogenous administration of GYY4137 (a slow release H2S releasing compound) on OTR expression in the heart, after acute on chronic disease, of CS exposed mice undergoing Txt.Methods: This study is a post hoc analysis of material obtained in wild type (WT) homozygous CSE−/− mice after 2-3 weeks of CS exposure and subsequent anesthesia, blast wave-induced TxT, and surgical instrumentation for mechanical ventilation (MV) and hemodynamic monitoring. CSE−/− animals received a 50 μg/g GYY4137-bolus after TxT. After 4h of MV, animals were exsanguinated and organs were harvested. The heart was cut transversally, formalin-fixed, and paraffin- embedded. Immunohistochemistry for OTR, arginine-vasopressin-receptor (AVPR), and vascular endothelial growth factor (VEGF) was performed with naïve animals as native controls.Results: CSE−/− was associated with hypertension and lower blood glucose levels, partially and significantly restored by GYY4137 treatment, respectively. Myocardial OTR expression was reduced upon injury, and this was aggravated in CSE−/−. Exogenous H2S administration restored myocardial protein expression to WT levels.Conclusions: This study suggests that cardiac CSE regulates cardiac OTR expression, and this effect might play a role in the regulation of cardiovascular function.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 323473
- DOI 10.1186/s40635-018-0207-0
- Persistent URL
- https://folia.unifr.ch/unifr/documents/307489
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