A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
- Septiadi, Dedy Adolphe Merkle Institute, University of Fribourg, Switzerland
- Bourquin, Joel Adolphe Merkle Institute, University of Fribourg, Switzerland
- Durantie, Estelle Adolphe Merkle Institute, University of Fribourg, Switzerland
- Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
- Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland
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29.06.2018
Published in:
- Scientific Reports. - 2018, vol. 8, no. 1, p. 9861
English
Three dimensional (3D) co-cultures to mimic cellular dynamics have brought significant impacts in tissue engineering approaches for biomedical research. Herein, we present a novel sample holder combined with time-lapse fluorescence imaging technique, referred as 4D live cell imaging, allowing direct visualization of various cells up to 24 hours. We further extended our approach to monitor kinetics and dynamics of particle uptake by cells and translocation across tissue membranes.
- Faculty
- Faculté des sciences et de médecine
- Department
- AMI - Bio-Nanomatériaux
- Language
-
- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 322807
- DOI 10.1038/s41598-018-28206-2
- Persistent URL
- https://folia.unifr.ch/unifr/documents/307387
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