Journal article

+ 1 other files

Arp3 controls the podocyte architecture at the kidney filtration barrier

  • Schell, Christoph Institute of Surgical Pathology, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany - Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany - Berta-Ottenstein Programme, Faculty of Medicine, University of Freiburg, Germany
  • Sabass, Benedikt Institute of Complex Systems-2, Forschungszentrum Jülich, Germany
  • Helmstaedter, Martin Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • Geist, Felix Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • Abed, Ahmed Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • Yasuda-Yamahara, Mako Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany - Department of Medicine, Shiga University of Medical Science, Otsu, Shiga Japan
  • Sigle, August Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • Maier, Jasmin I. Institute of Surgical Pathology, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany - Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • Grahammer, Florian Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany - Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Siegerist, Florian Department of Anatomy and Cell Biology, University Medicine Greifswald, Germany
  • Artelt, Nadine Department of Anatomy and Cell Biology, University Medicine Greifswald, Germany
  • Endlich, Nicole Department of Anatomy and Cell Biology, University Medicine Greifswald, Germany
  • Kerjaschki, Dontscho Department of Pathology, Medical University of Vienna, Austria
  • Arnold, Hans-Henning Cell and Molecular Biology, Technical University of Braunschweig, Germany
  • Dengjel, Jörn BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-University Freiburg, Germany - Department of Biology, University of Fribourg, Switzerland - Department of Dermatology, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • Rogg, Manuel Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany -
  • Huber, Tobias B. Department of Medicine IV, Medical Center – University of Freiburg, Faculty of Medicine, University of Freiburg, Germany - Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany - BIOSS Center for Biological Signaling Studies, Albert-Ludwigs-University Freiburg, Germany -
Show more…
    17.12.2018
Published in:
  • Developmental Cell. - 2018, vol. 47, no. 6, p. 741-757.e8
English Podocytes, highly specialized epithelial cells, build the outer part of the kidney filtration barrier and withstand high mechanical forces through a complex network of cellular protrusions. Here, we show that Arp2/3-dependent actin polymerization controls actomyosin contractility and focal adhesion maturation of podocyte protrusions and thereby regulates formation, maintenance, and capacity to adapt to mechanical requirements of the filtration barrier. We find that N-WASP-Arp2/3 define the development of complex arborized podocyte protrusions in vitro and in vivo. Loss of dendritic actin networks results in a pronounced activation of the actomyosin cytoskeleton and the generation of over-maturated but less efficient adhesion, leading to detachment of podocytes. Our data provide a model to explain podocyte protrusion morphology and their mechanical stability based on a tripartite relationship between actin polymerization, contractility, and adhesion.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307333
Other files

Statistics

Document views: 20 File downloads:
  • den_acp.pdf: 35
  • den_acp_sm1.pdf: 20