Single exposure to aerosolized graphene oxide and graphene nanoplatelets did not initiate an acute biological response in a 3D human lung model

Drasler, Barbara; Kucki, Melanie; Delhaes, Flavien; Buerki-Thurnherr, Tina; Vanhecke, Dimitri; Korejwo, Daria; Chortare, Savvina; Barosova, Hana; Hirsch, Cordula; Petri-Fink, Alke; Rothen-Rutishauser, Barbara; Wick, Peter

doi:10.1016/j.carbon.2018.05.012

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Single exposure to aerosolized graphene oxide and graphene nanoplatelets did not initiate an acute biological response in a 3D human lung model

Drasler, Barbara BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland
Kucki, Melanie Laboratory for Particles-Biology Interactions, EMPA - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland
Delhaes, Flavien BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland
Buerki-Thurnherr, Tina Laboratory for Particles-Biology Interactions, EMPA - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland
Vanhecke, Dimitri BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland
Korejwo, Daria Laboratory for Particles-Biology Interactions, EMPA - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland
Chortare, Savvina BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland - Laboratory for Particles-Biology Interactions, EMPA - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland
Barosova, Hana BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland
Hirsch, Cordula Laboratory for Particles-Biology Interactions, EMPA - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland
Petri-Fink, Alke BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
Rothen-Rutishauser, Barbara BioNanomaterials Group, Adolphe Merkle Institute, University of Fribourg, Switzerland
Wick, Peter Laboratory for Particles-Biology Interactions, EMPA - Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland

01.10.2018

Published in:

Carbon. - 2018, vol. 137, p. 125–135

English The increased mass production of graphene related materials (GRM), intended for a broad spectrum of applications, demands a thorough assessment of their potential hazard to humans and the environment. Particularly, the paramount concern has been expressed in regard to their interaction with the respiratory system in occupational exposure settings. It has been shown that GRM are easily respirable and can interact with lung cells resulting in the induction of oxidative stress or pulmonary inflammation. However, a comprehensive assessment of potential biological effects induced by GRM is currently hardly feasible to accomplish due to the lack of well-defined GRM materials and realistic exposure data. Herein, a 3D human lung model was combined with a commercial aerosolization system to study potential side effects of GRM. Two representative types of GRM were aerosolized onto the lung epithelial tissue surface. After 24 h post exposure, selected biological endpoints were evaluated, such as cell viability, morphology, barrier integrity, induction of (pro-)inflammation and oxidative stress reactions and compared with the reference material carbon black. Single exposure to all tested GRM at the two different exposure concentrations (∼300 and 1000 ng/cm2) did not initiate an observable adverse effect to the 3D lung model under acute exposure scenarios.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie, AMI - Bio-Nanomatériaux

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