Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

Chortarea, Savvina; Fytianos, Kleanthis; Rodriguez-Lorenzo, Laura; Petri-Fink, Alke; Rothen-Rutishauser, Barbara

doi:10.2217/nnm-2017-0358

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Distribution of polymer-coated gold nanoparticles in a 3D lung model and indication of apoptosis after repeated exposure

Chortarea, Savvina BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Switzerland - Laboratory for Particles ? Biology Interactions, Empa, Swiss Federal Laboratories for Materials, Science & Technology, St Gallen, Switzerland
Fytianos, Kleanthis BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Pulmonary Medicine, University of Bern, Switzerland - Department of Clinical Research, University of Bern, Switzerland
Rodriguez-Lorenzo, Laura BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Switzerland
Petri-Fink, Alke BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
Rothen-Rutishauser, Barbara BioNanomaterials, Adolphe Merkle Institute, University of Fribourg, Switzerland

01.05.2018

Published in:

Nanomedicine. - 2018, vol. 13, no. 10, p. 1169–1185

English Aim: The distribution and impact of aerosol-delivered gold nanoparticles (AuNPs) functionalized with a mixture of aminated-polyvinyl alcohol and amino-PEG ([polyvinyl alcohol/PEG]-NH2) upon repeated administration onto a 3D lung model were explored. Materials & methods: AuNPs were aerosolized and uptake and epithelial translocation was assessed by inductively coupled plasma optical-emission spectroscopy, flow cytometry and electron microscopy. In addition, cytotoxicity, apoptosis and proinflammation were evaluated. Results: Repeated AuNP aerosolization resulted in NP accumulation in macrophages and epithelial cells. Dendritic cells demonstrated substantial NP internalization after single administration which was reduced in later time points. No cytotoxicity or proinflammation was observed but after 96 h significant apoptosis was induced by the polymer coating. Conclusion: These results indicate the importance of repeated exposures in addressing potential effects of NPs.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie

Language