Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil

Kieffer, Nicolas; Nordmann, Patrice; Moreno, Andrea Micke; Zanolli Moreno, Luisa; Chaby, Richard; Breton, Aude; Tissières, Pierre; Poirel, Laurent

doi:10.1128/AAC.00278-18

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Journal article

Genetic and Functional Characterization of an MCR-3-Like Enzyme-Producing Escherichia coli Isolate Recovered from Swine in Brazil

Kieffer, Nicolas Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland
Nordmann, Patrice Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
Moreno, Andrea Micke Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, Brazil - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland
Zanolli Moreno, Luisa Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, Brazil
Chaby, Richard Institute for integrative biology of the cell, Université Paris-Sud & Paris-Saclay, Orsay, France
Breton, Aude Institute for integrative biology of the cell, Université Paris-Sud & Paris-Saclay, Orsay, France
Tissières, Pierre Institute for integrative biology of the cell, Université Paris-Sud & Paris-Saclay, Orsay, France
Poirel, Laurent Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland

01.07.2018

Published in:

Antimicrobial Agents and Chemotherapy. - 2018, vol. 62, no. 7, p. e00278-18

English A collection of 126 pigs was screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eight colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a function similar to that of MCR-1 and MCR-2 as a result of the addition of a phosphoethanolamine group to the lipid A moiety. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C2 plasmid and was embedded in an original genetic environment. This study reports the occurrence of the MCR-3-like determinant in South America and is the first to demonstrate the functionality of this group of enzymes as a phosphoethanolamine transferase.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language