A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport

Zhang, Yuqin; Nasser, Victoria; Pisanty, Odelia; Omary, Moutasem; Wulff, Nikolai; Donato, Martin Di; Tal, Iris; Hauser, Felix; Hao, Pengchao; Roth, Ohad; Fromm, Hillel; Schroeder, Julian I.; Geisler, Markus; Nour-Eldin, Hussam Hassan; Shani, Eilon

doi:10.1038/s41467-018-06410-y

Back

Journal article

+ 1 other files

A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport

Zhang, Yuqin School of Plant Sciences and Food Security, Tel Aviv University, Israel
Nasser, Victoria School of Plant Sciences and Food Security, Tel Aviv University, Israel
Pisanty, Odelia School of Plant Sciences and Food Security, Tel Aviv University, Israel
Omary, Moutasem School of Plant Sciences and Food Security, Tel Aviv University, Israel
Wulff, Nikolai DynaMo Center, Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark
Donato, Martin Di Department of Biology, University of Fribourg, Switzerland
Tal, Iris School of Plant Sciences and Food Security, Tel Aviv University, Israel
Hauser, Felix Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, USA
Hao, Pengchao Department of Biology, University of Fribourg, Switzerland
Roth, Ohad School of Plant Sciences and Food Security, Tel Aviv University, Israel
Fromm, Hillel School of Plant Sciences and Food Security, Tel Aviv University, Israel
Schroeder, Julian I. Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, USA
Geisler, Markus Department of Biology, University of Fribourg, Switzerland
Nour-Eldin, Hussam Hassan DynaMo Center, Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark
Shani, Eilon School of Plant Sciences and Food Security, Tel Aviv University, Israel

11.10.2018

Published in:

Nature Communications. - 2018, vol. 9, no. 1, p. 4204

English Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial- distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language