A transportome-scale amiRNA-based screen identifies redundant roles of Arabidopsis ABCB6 and ABCB20 in auxin transport
- Zhang, Yuqin School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Nasser, Victoria School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Pisanty, Odelia School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Omary, Moutasem School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Wulff, Nikolai DynaMo Center, Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark
- Donato, Martin Di Department of Biology, University of Fribourg, Switzerland
- Tal, Iris School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Hauser, Felix Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, USA
- Hao, Pengchao Department of Biology, University of Fribourg, Switzerland
- Roth, Ohad School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Fromm, Hillel School of Plant Sciences and Food Security, Tel Aviv University, Israel
- Schroeder, Julian I. Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, USA
- Geisler, Markus Department of Biology, University of Fribourg, Switzerland
- Nour-Eldin, Hussam Hassan DynaMo Center, Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark
- Shani, Eilon School of Plant Sciences and Food Security, Tel Aviv University, Israel
-
11.10.2018
Published in:
- Nature Communications. - 2018, vol. 9, no. 1, p. 4204
English
Transport of signaling molecules is of major importance for regulating plant growth, development, and responses to the environment. A prime example is the spatial- distribution of auxin, which is regulated via transporters to govern developmental patterning. A critical limitation in our ability to identify transporters by forward genetic screens is their potential functional redundancy. Here, we overcome part of this functional redundancy via a transportome, multi-targeted forward-genetic screen using artificial-microRNAs (amiRNAs). We generate a library of 3000 plant lines expressing 1777 amiRNAs, designed to target closely homologous genes within subclades of transporter families and identify, genotype and quantitatively phenotype, 80 lines showing reproducible shoot growth phenotypes. Within this population, we discover and characterize a strong redundant role for the unstudied ABCB6 and ABCB20 genes in auxin transport and response. The unique multi-targeted lines generated in this study could serve as a genetic resource that is expected to reveal additional transporters.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
-
- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
-
- RERO DOC 323291
- DOI 10.1038/s41467-018-06410-y
- Persistent URL
- https://folia.unifr.ch/unifr/documents/307088
Other files
Statistics
Document views: 24
File downloads:
- gei_tsa.pdf: 32
- gei_tsa_sm.pdf: 36