Journal article

Influenza A virus induces autophagosomal targeting of ribosomal proteins

  • Becker, Andrea C. Department of Dermatology, Medical Center University of Freiburg, Germany - Faculty of Medicine, University of Freiburg, Germany
  • Gannagé, Monique Department of Pathology and Immunology, School of Medicine, University of Geneva, Switzerland
  • Giese, Sebastian Faculty of Medicine, University of Freiburg, Germany - Institute for Virology, Medical Center, University of Freiburg, Germany
  • Hu, Zehan Department of Dermatology, Medical Center University of Freiburg, Germany - Faculty of Medicine, University of Freiburg, Germany - Department of Biology, University of Fribourg, Switzerland
  • Abou-Eid, Shadi Department of Biology, University of Fribourg, Switzerland
  • Roubaty, Carole Department of Biology, University of Fribourg, Switzerland
  • Paul, Petra Viral Immunobiology, Institute of Experimental Immunology, University of Zurich, Switzerland
  • Bühler, Lea Department of Dermatology, Medical Center University of Freiburg, Germany - Faculty of Medicine, University of Freiburg, Germany
  • Gretzmeier, Christine Department of Dermatology, Medical Center University of Freiburg, Germany - Faculty of Medicine, University of Freiburg, Germany
  • Dumit, Veronica I. Core Facility Proteomics, Center for Biological Systems Analysis (ZBSA), University of Freiburg, Germany
  • Kaeser-Pebernard, Stéphanie Department of Biology, University of Fribourg, Switzerland
  • Schwemmle, Martin Faculty of Medicine, University of Freiburg, Germany
  • Münz, Christian Viral Immunobiology, Institute of Experimental Immunology, University of Zurich, Switzerland
  • Dengjel, Jörn Department of Dermatology, Medical Center University of Freiburg, Germany - Faculty of Medicine, University of Freiburg, Germany - Department of Biology, University of Fribourg, Switzerland - Department of Pathology and Immunology, School of Medicine, University of Geneva, Switzerland
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    01.10.2018
Published in:
  • Molecular & Cellular Proteomics. - 2018, vol. 17, no. 10, p. 1909–1921
English Seasonal epidemics of influenza A virus are a major cause of severe illness and are of high socio-economic relevance. For the design of effective antiviral therapies, a detailed knowledge of pathways perturbed by virus infection is critical. We performed comprehensive expression and organellar proteomics experiments to study the cellular consequences of influenza A virus infection using three human epithelial cell lines derived from human lung carcinomas: A549, Calu-1 and NCI-H1299. As a common response, the type I interferon pathway was up-regulated upon infection. Interestingly, influenza A virus infection led to numerous cell line-specific responses affecting both protein abundance as well as subcellular localization. In A549 cells, the vesicular compartment appeared expanded after virus infection. The composition of autophagsomes was altered by targeting of ribosomes, viral mRNA and proteins to these double membrane vesicles. Thus, autophagy may support viral protein translation by promoting the clustering of the respective molecular machinery in autophagosomes in a cell line-dependent manner.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/307036
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