Journal article

Identification of general patterns of sex-biased expression in Daphnia, a genus with environmental sex determination

  • Molinier, Cécile CEFE, Centre National de la Recherche Scientifique, Université de Montpellier? EPHE, Montpellier, France - Université de Fribourg, Ecology and Evolution, Switzerland
  • Reisser, Céline M. O. CEFE, Centre National de la Recherche Scientifique, Université de Montpellier? EPHE, Montpellier, France - Université de Fribourg, Ecology and Evolution, Switzerland
  • Fields, Peter Universität Basel, Zoology Institute, Evolutionary Biology, Switzerland
  • Ségard, Adeline CEFE, Centre National de la Recherche Scientifique, Université de Montpellier? EPHE, Montpellier, France
  • Galimov, Yan Koltsov Institute of Developmental Biology, Moscow, Russia
  • Haag, Christoph R. CEFE, Centre National de la Recherche Scientifique, Université de Montpellier? EPHE, Montpellier, France - Université de Fribourg, Ecology and Evolution, Switzerland
Show more…
    04.03.2018
Published in:
  • G3& Genes|Genomes|Genetics. - 2018, vol. 8, no. 5, p. 1523–1533
English Daphnia reproduce by cyclic-parthenogenesis, where phases of asexual reproduction are intermitted by sexual production of diapause stages. This life cycle, together with environmental sex determination, allow the comparison of gene expression between genetically identical males and females. We investigated gene expression differences between males and females in four genotypes of Daphnia magna and compared the results with published data on sex-biased gene expression in two other Daphnia species, each representing one of the major phylogenetic clades within the genus. We found that 42% of all annotated genes showed sex-biased expression in D. magna. This proportion is similar both to estimates from other Daphnia species as well as from species with genetic sex determination, suggesting that sex-biased expression is not reduced under environmental sex determination. Among 7453 single copy, one-to-one orthologs in the three Daphnia species, 707 consistently showed sex-biased expression and 675 were biased in the same direction in all three species. Hence these genes represent a core-set of genes with consistent sex-differential expression in the genus. A functional analysis identified that several of them are involved in known sex determination pathways. Moreover, 75% were overexpressed in females rather than males, a pattern that appears to be a general feature of sex-biased gene expression in Daphnia.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306846
Statistics

Document views: 20 File downloads:
  • haa_igp.pdf: 39