NAB2 is a novel immune stimulator of MDA-5 that promotes a strong type I interferon response
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Oberson, Anne
Pharmacology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
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Spagnuolo, Lorenzo
Pharmacology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - School of Pharmaceutical Sciences, University of Geneva, Switzerland - Department of Anesthesiology, Pharmacology and Intensive Care, Faculty of Medicine, University of Geneva, Switzerland
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Puddinu, Viola
School of Pharmaceutical Sciences, University of Geneva, Switzerland - Department of Anesthesiology, Pharmacology and Intensive Care, Faculty of Medicine, University of Geneva, Switzerland
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Barchet, Winfried
German Center for Infection Research, Cologne-Bonn, Germany - Institute for Clinical Chemistry and Clinical Pharmacology, University of Bonn, Germany
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Rittner, Karola
Transgene S.A., Parc d?Innovation, Illkirch-Graffenstaden, France
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Bourquin, Carole
Pharmacology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - School of Pharmaceutical Sciences, University of Geneva, Switzerland - Department of Anesthesiology, Pharmacology and Intensive Care, Faculty of Medicine, University of Geneva, Switzerland
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Published in:
- Oncotarget. - 2018, vol. 9, no. 5, p. 5641-5651
English
Novel adjuvants are needed to increase the efficacy of vaccine formulations and immune therapies for cancer and chronic infections. In particular, adjuvants that promote a strong type I IFN response are required, since this cytokine is crucial for the development of efficient anti-tumoral and anti-viral immunity. Nucleic acid band 2 (NAB2) is a double- stranded RNA molecule isolated from yeast and identified as an agonist of the pattern- recognition receptors TLR3 and MDA-5. We compared the ability of NAB2 to activate innate immunity with that of poly(I:C), a well-characterized TLR3 and MDA-5 agonist known for the induction of type I IFN. NAB2 promoted stronger IFN-α production and induced a higher activation state of both murine and human innate immune cells compared to poly(I:C). This correlated with a stronger activation of the signalling pathway downstream of MDA-5, and IFN-α induction was dependent on MDA-5. Upon injection, NAB2 induced higher levels of serum IFN-α in mice than poly(I:C). These results suggest that NAB2 has the potential to become an efficient adjuvant for the induction of type-I IFN responses in therapeutic immunization against cancer or infections.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Médecine
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/306653
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