Integrase-mediated recombination of the bel-1 gene cassette encoding the extended-spectrum β-lactamase BEL-1
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Bouheraoua, Nacim
Quinze-Vingts National Ophthalmology Hospital, UPMC-Sorbonne Universities, Paris, France
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Poirel, Laurent
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, 3INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
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Bourreau, Baptiste
EA7361, Université Paris-Sud, Université Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene unit, APHP, Hôpital Bicêtre, Le Kremlin-Bicêtre, France - EERA ?Evolution and Ecology of Resistance to Antibiotics? Unit, Institut Pasteur-APHP-Université Paris Sud, Paris, France - Associated French National Reference Center for Antibiotic Resistance ?Carbapenemase-producing Enterobacteriaceae?, University of Lausanne, Switzerland
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Bonnin, Rémy
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, 3INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
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Laroche, Laurent
Quinze-Vingts National Ophthalmology Hospital, UPMC-Sorbonne Universities, Paris, France
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Naas, Thierry
EA7361, Université Paris-Sud, Université Paris-Saclay, LabEx Lermit, Bacteriology-Hygiene unit, APHP, Hôpital Bicêtre, Le Kremlin-Bicêtre, France - EERA ?Evolution and Ecology of Resistance to Antibiotics? Unit, Institut Pasteur-APHP-Université Paris Sud, Paris, France - Associated French National Reference Center for Antibiotic Resistance ?Carbapenemase-producing Enterobacteriaceae?, University of Lausanne, Switzerland
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Nordmann, Patrice
Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, 3INSERM European Unit (IAME, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - Institute for Microbiology, University of Lausanne and University Hospital Centre, Lausanne, Switzerland
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Published in:
- Antimicrobial Agents and Chemotherapy. - 2018, p. AAC.00030-18
English
Integrons are genetic elements that can acquire and rearrange gene cassettes. The blaBEL-1 gene encodes an extended-spectrum β-lactamase, BEL-1, that is present at second position of the variable region of class 1 integrons identified in Pseudomonas aeruginosa. The mobility of the bel-1 gene cassette was analyzed in physiological conditions and with the integrase gene being overexpressed. Cassette mobility in Escherichia coli was detected by excision/integration into the recipient integron In3 on the conjugative plasmid R388, with the over-produced integrase. Despite several antibiotic pressures, the bel-1 cassette remained at the second position in the integron, highlighting its stability in P. aeruginosa. Overexpression of the integrase gene in E. coli induced the bel-1 cassette recombination. However, cassettes containing two genes (blaBEL-1/smr2 or blaBEL-1/aacA4) were excised suggesting that the bel-1 cassette attC site was defective. We showed that bel-1 is a stable gene cassette under physiological growth conditions irrespective of the selective antibiotic pressure, that may be mobilized upon over-overexpression of the integrase gene.
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Faculty
- Faculté des sciences et de médecine
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Department
- Médecine 3ème année
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/306597
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