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Facile and rapid formation of giant vesicles from glass beads

  • Tanasescu, Radu Department of Chemistry, University of Fribourg, Switzerland
  • Mettal, Ute Department of Chemistry, University of Fribourg, Switzerland
  • Colom, Adai Department of Biochemistry, University of Geneva, Switzerland - National Centre of Competence in Research in Chemical Biology, Geneva, Switzerland
  • Roux, Aurélien Department of Biochemistry, University of Geneva, Switzerland - National Centre of Competence in Research in Chemical Biology, Geneva, Switzerland
  • Zumbuehl, Andreas Department of Chemistry, University of Fribourg, Switzerland - National Centre of Competence in Research in Chemical Biology, Geneva, Switzerland
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    09.01.2018
Published in:
  • Polymers. - 2018, vol. 10, no. 1, p. 54
English Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306541
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