Facile and rapid formation of giant vesicles from glass beads

Tanasescu, Radu; Mettal, Ute; Colom, Adai; Roux, Aurélien; Zumbuehl, Andreas

doi:10.3390/polym10010054

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Facile and rapid formation of giant vesicles from glass beads

Tanasescu, Radu Department of Chemistry, University of Fribourg, Switzerland
Mettal, Ute Department of Chemistry, University of Fribourg, Switzerland
Colom, Adai Department of Biochemistry, University of Geneva, Switzerland - National Centre of Competence in Research in Chemical Biology, Geneva, Switzerland
Roux, Aurélien Department of Biochemistry, University of Geneva, Switzerland - National Centre of Competence in Research in Chemical Biology, Geneva, Switzerland
Zumbuehl, Andreas Department of Chemistry, University of Fribourg, Switzerland - National Centre of Competence in Research in Chemical Biology, Geneva, Switzerland

09.01.2018

Published in:

Polymers. - 2018, vol. 10, no. 1, p. 54

English Giant vesicles (GVs) are widely-used model systems for biological membranes. The formulation of these vesicles, however, can be problematic and artifacts, such as degraded molecules or left-over oil, may be present in the final liposomes. The rapid formulation of a high number of artifact-free vesicles of uniform size using standard laboratory equipment is, therefore, highly desirable. Here, the gentle hydration method of glass bead-supported thin lipid films has been enhanced by adding a vortexing step. This led to the formulation of a uniform population of giant vesicles. Batches of glass beads coated with different lipids can be combined to produce vesicles of hybrid lipid compositions. This method represents a stable approach to rapidly generate giant vesicles.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie

Language