Complete genome sequencing of Acinetobacter baumannii str. K50 disclosed the large conjugative plasmid pK50a encoding the carbapenemase OXA-23 and the extended-spectrum β–lactamase GES-11

Wibberg, Daniel; Salto, Ileana P.; Eikmeyer, Felix G.; Maus, Irena; Winkler, Anika; Nordmann, Patrice; Pühler, Alfred; Poirel, Laurent; Schlüter, Andreas

doi:10.1128/AAC.00212-18

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Complete genome sequencing of Acinetobacter baumannii str. K50 disclosed the large conjugative plasmid pK50a encoding the carbapenemase OXA-23 and the extended-spectrum β–lactamase GES-11

Wibberg, Daniel Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Germany
Salto, Ileana P. IBBM, CCT-CONICET-La Plata, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina
Eikmeyer, Felix G. von Laer Stiftung, Bielefeld, Germany
Maus, Irena Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Germany
Winkler, Anika Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Germany - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland - HFR - Hôpital Cantonal, Fribourg, Switzerland
Pühler, Alfred Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Germany
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, France), University of Fribourg, Switzerland - Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Switzerland
Schlüter, Andreas Genome Research of Industrial Microorganisms, Center for Biotechnology (CeBiTec), Bielefeld University, Germany

20.02.2018

Published in:

Antimicrobial Agents and Chemotherapy. - 2018, p. AAC.00212-18

English Multidrug-resistant (MDR) Acinetobacter baumannii strains appeared as serious emerging nosocomial pathogens in clinical environments and especially in intensive care units (ICUs). A. baumannii strain K50 recovered from a hospitalized patient in Kuwait exhibited resistance to carbapenems, and additionally to ciprofloxacin, chloramphenicol, sulfonamides, amikacin and gentamicin. Genome sequencing revealed that the strain possesses two plasmids, pK50a (79.6 kb) and pK50b (9.5 kb) and a 3.75 Mb chromosome. A. baumannii K50 exhibits an Average Nucleotide Identity (ANI) value of 99.98% to the previously reported Iraqi clinical isolate AA-014, even though that the latter strain lacked plasmid pK50a. Strain K50 belongs to the Sequence Type ST158 (Pasteur scheme) and ST499 according to the Oxford scheme. Plasmid pK50a is a member of the Aci6 (RG6) group of Acinetobacter plasmids, and encodes a conjugative transfer module and two antibiotic resistance gene regions comprising the transposon Tn2008. The transposon carries the carbapenemase gene blaOXA-23 and a class 1 integron harboring the resistance genes blaGES-11, aacA4, dfrA7, qacEΔ1 and sul1 conferring resistance to all β- lactams and reduced susceptibility to carbapenems, resistance to aminoglycosides, trimethoprim, quaternary ammonium compounds and sulfamethoxazole, respectively. The class 1 integron is flanked by MITEs (Miniature Inverted repeat Transposable Element) delimiting the element at its insertion site.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language