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Are animals a source of Stenotrophomonas maltophilia in human infections? Contributions of a nationwide molecular study

  • Jayol, Aurélie Department of Microbiology, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France - Emerging Antibiotic Resistance Unit, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA-IAME Paris, France), University of Fribourg, Switzerland
  • Corlouer, Camille Department of Microbiology, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France
  • Haenni, Marisa Unité Antibiorésistance et Virulence Bactériennes, ANSES, Lyon, France
  • Darty, Mélanie Next Generation Sequencing Platform, University Hospital Henri Mondor, Assistance Publique-Hôpitaux de Paris, Créteil, France
  • Maillard, Karine Laboratoire Labeo Frank Duncombe, Caen, France
  • Desroches, Marine Department of Microbiology, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France
  • Lamy, Brigitte Department of Bacteriology, Archet 2 Hospital, Nice Academic Hospital, Nice, France - INSERM U1065, C3M, Team 6, Nice, France
  • Jumas-Bilak, Estelle Infection Control Department, Montpellier University Hospital, Montpellier, France - UMR5569 HydroSciences Montpellier, Faculté de Pharmacie, Montpellier, France
  • Madec, Jean-Yves Unité Antibiorésistance et Virulence Bactériennes, ANSES, Lyon, France
  • Decousser, Jean-Winoc Department of Microbiology, Assistance Publique-Hôpitaux de Paris, University Hospital Henri Mondor, Créteil, France - EA 7380 Dynamyc Université Paris-Est Créteil (UPEC), Ecole nationale vétérinaire d?Alfort (EnvA), Créteil, France
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    27.02.2018
Published in:
  • European Journal of Clinical Microbiology & Infectious Diseases. - 2018, p. 1–7
English Stenotrophomonas maltophilia (Sm) is an archetypal environmental opportunistic bacterium responsible for health care-associated infections. The role of animals in human Sm infections is unknown. This study aims to reveal the genetic and phylogenetic relationships between pathogenic strains of Sm, both animal and human, and identify a putative role for animals as a reservoir in human infection. We phenotypically and genotypically characterized 61 Sm strains responsible for animal infections (mainly respiratory tract infections in horses) from a French nationwide veterinary laboratory network. We tested antimicrobial susceptibility and performed MLST and genogrouping using the concatenation of the seven housekeeping genes from the original MLST scheme. Excluding the eight untypeable strains owing to the lack of gene amplification, only 10 out of the 53 strains yielded a known ST (ST5, ST39, ST162, ST8, ST27, ST126, ST131). The genogroup distribution highlighted not only genogroups (genogroups 5 and 9) comprised exclusively of animal strains but also genogroups shared by human and animal strains. Interestingly, these shared genogroups were primarily groups 2 and 6, which have previously been identified as the two most frequent genogroups among human-pathogenic Sm strains, especially among respiratory pathogens. The antimicrobial susceptibility testing underlined the presence of acquired resistance: 18.8 and 7.5% of the tested isolates were resistant to the sulfonamide-trimethoprim combination and ciprofloxacin, respectively. Animal strains of Sm shared phylogenetic traits with some of the most successful human strains. The exact relationships between the human and animal strains, and the genetic support of these common traits, need to be determined.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306523
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