Journal article

Hafnia, an enterobacterial genus naturally resistant to colistin revealed by three susceptibility testing methods

  • Jayol, Aurélie Laboratory of Bacteriology, University Hospital of Bordeaux, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France - Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland
  • Saly, Marion Laboratory of Bacteriology, University Hospital of Bordeaux, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - University Hospital and University of Lausanne, Switzerland
  • Ménard, Armelle Laboratory of Bacteriology, University Hospital of Bordeaux, Bordeaux, France - UMR1053, Bordeaux Research in Translational Oncology BaRITOn, University of Bordeaux, France
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland
  • Dubois, Véronique Laboratory of Bacteriology, University Hospital of Bordeaux, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France
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    01.09.2017
Published in:
  • Journal of Antimicrobial Chemotherapy. - 2017, vol. 72, no. 9, p. 2507–2511
English ObjectivesTo determine the susceptibility to colistin of Hafnia alvei and Hafnia paralvei, and to compare methods for colistin resistance detection in the Hafnia genus.MethodsA collection of 25 Hafnia isolates was studied. Species were identified by using 16S rRNA gene sequencing with subsequent phylogeny analysis. Susceptibility to colistin was determined using the broth microdilution (BMD) reference method, the Phoenix automated system, the Rapid Polymyxin NP test, the Etest system and the disc diffusion method.ResultsThe collection consisted of 15 H. alvei and 10 H. paralvei isolates. Based on the 16S rRNA analysis, a close relationship of the Hafnia genus with naturally colistin-resistant enterobacterial genera (Proteus, Morganella, Providencia and Serratia) was identified. Susceptibility testing performed using the BMD method, the Phoenix automated system and the Rapid Polymyxin NP test revealed a high rate of colistin resistance (96%). Underestimation of colistin resistance using Etest strips (72%) and the disc diffusion method (0%) was observed.ConclusionsThe high rate of colistin resistance observed within the Hafnia genus and its close phylogenetic relationship with naturally colistin-resistant genera suggest that Hafnia is a naturally colistin-resistant enterobacterial genus.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306456
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