Journal article

Myocardial infarction does not affect circulating haematopoietic stem and progenitor cell self‐renewal ability in a rat model

  • Kröpfl, J. M. Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich, Switzerland
  • Spengler, C. M. Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, ETH Zurich, Switzerland - Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Switzerland
  • Frobert, Aurélien Cardiology, University of Fribourg, Switzerland
  • Ajalbert, Guillaume Cardiology, University of Fribourg, Switzerland
  • Giraud, Marie-Noëlle Cardiology, University of Fribourg, Switzerland
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  • Experimental Physiology. - 2018, vol. 103, no. 1, p. 1–8
English Given the importance of peripheral blood haematopoietic stem and progenitor cells (HPCs) in post-acute regeneration after acute myocardial infarction (MI), the aim of the present study was to investigate the number and secondary replating capacity/self-renewal ability of HPCs in peripheral blood before and 2 weeks after MI. In female Lewis inbred rats (n = 9), MI was induced by ligation of the left coronary artery, and another nine underwent sham surgery, without ligation, for control purposes. Myocardial infarction was confirmed by troponin I concentrations 24 h after surgery. Peripheral blood was withdrawn and fractional shortening and ejection fraction of the left ventricle were assessed before (day 0) and 14 days after MI or sham surgery (day 14). After mononuclear cell isolation, primary and secondary functional colony-forming unit granulocyte–macrophage (CFU-GM) assays were performed in order to detect the kinetics of functional HPC colony counts and cell self- renewal ability in vitro. The CFU-GM counts and cell self-renewal ability remained unchanged (P < 0.05) in both groups at day 14, without interaction between groups. In the intervention group, higher day 0 CFU-GM counts showed a relationship to lower fractional shortening on day 14 (ρ = −0.82; P < 0.01). Myocardial infarction did not negatively affect circulating HPC self-renewal ability, which suggests a constant regenerative potential in the post-acute phase. A relationship of cardiac contractile function 14 days after MI with circulating CFU-GM counts on day 0 might imply functional colony count as a predictive factor for outcome after infarction.
Faculté des sciences et de médecine
Médecine 3ème année
  • English
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