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Solid‐phase synthesis of the aged‐nonapeptide‐nerve‐agent adduct of butyrylcholinesterase as reference materials for analytical verification
Spiez Laboratory, Spiez, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
Spiez Laboratory, Spiez, Switzerland
Bochet, Christian G.
Department of Chemistry, University of Fribourg, Switzerland
- Helvetica Chimica Acta. - 2017, vol. 100, no. 11, p. e1700198
Two pathways were developed and investigated for the synthesis of the ‘aged’- nonapeptide nerve-agent bioadduct of human butyrylcholinesterase (BuChE). Considering the fast ageing of nerve-agent adducts of BuChE in patients and biomedical samples this target molecule is of paramount relevance for quantitative analysis with respect to the Chemical Weapons Convention. Two approaches using a precursor bearing a hydroxyl on its phosphonyl moiety and a benzyl protected precursor were considered. Several impurities were identified and circumvented during the optimization of the peptide synthesis step. The ‘aged’-nonapeptide adduct was successfully synthesized by solid-phase-peptide-synthesis (SPPS).
- Faculté des sciences et de médecine
- Département de Chimie
- boc_sps.pdf: 8
- boc_sps_sm.pdf: 3