Journal article

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Correlation of surface pressure and hue of planarizable push-pull chromophores at the air/water interface

  • Neuhaus, Frederik Department of Chemistry, University of Fribourg, Switzerland - National Centre of Competence in Research (NCCR) Chemical Biology, Geneva, Switzerland
  • Zobi, Fabio Department of Chemistry, University of Fribourg, Switzerland
  • Brezesinski, Gerald Max Planck Institute of Colloids and Interfaces, Science Park Potsdam-Golm, Potsdam, Germany
  • Molin, Marta Dal National Centre of Competence in Research (NCCR) Chemical Biology, Geneva, Switzerland -
  • Matile, Stefan National Centre of Competence in Research (NCCR) Chemical Biology, Geneva, Switzerland -
  • Zumbuehl, Andreas Department of Chemistry, University of Fribourg, Switzerland - National Centre of Competence in Research (NCCR) Chemical Biology, Geneva, Switzerland
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    08.06.2017
Published in:
  • Beilstein Journal of Organic Chemistry. - 2017, vol. 13, no. 1, p. 1099–1105
English It is currently not possible to directly measure the lateral pressure of a biomembrane. Mechanoresponsive fluorescent probes are an elegant solution to this problem but it requires first the establishment of a direct correlation between the membrane surface pressure and the induced color change of the probe. Here, we analyze planarizable dithienothiophene push–pull probes in a monolayer at the air/water interface using fluorescence microscopy, grazing-incidence angle X-ray diffraction, and infrared reflection–absorption spectroscopy. An increase of the lateral membrane pressure leads to a well-packed layer of the ‘flipper’ mechanophores and a clear change in hue above 18 mN/m. The fluorescent probes had no influence on the measured isotherm of the natural phospholipid DPPC suggesting that the flippers probe the lateral membrane pressure without physically changing it. This makes the flipper probes a truly useful addition to the membrane probe toolbox.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306132
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