High-level resistance to colistin mediated by various mutations in the crrb gene among carbapenemase-producing klebsiella pneumoniae
- Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, University of Fribourg, Switzerland - Laboratory of Bacteriology, Bordeaux University Hospital, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France
- Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, University of Fribourg, Switzerland - University of Lausanne and University Hospital Center, Lausanne, Switzerland
- Brink, Adrian Department of Clinical Microbiology, Ampath National Laboratory Services, Milpark Hospital, Johannesburg, South Africa
- Villegas, Maria-Virginia International Center for Medical Research and Training, CIDEIM, Cali, Colombia
- Dubois, Véronique Laboratory of Bacteriology, Bordeaux University Hospital, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France
- Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, University of Fribourg, Switzerland
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11.01.2017
Published in:
- Antimicrobial Agents and Chemotherapy. - 2017, vol. 61, no. 11, p. e01423-17
English
Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin- resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 305752
- DOI 10.1128/AAC.01423-17
- Persistent URL
- https://folia.unifr.ch/unifr/documents/306106
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