Journal article

High-level resistance to colistin mediated by various mutations in the crrb gene among carbapenemase-producing klebsiella pneumoniae

  • Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, University of Fribourg, Switzerland - Laboratory of Bacteriology, Bordeaux University Hospital, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, University of Fribourg, Switzerland - University of Lausanne and University Hospital Center, Lausanne, Switzerland
  • Brink, Adrian Department of Clinical Microbiology, Ampath National Laboratory Services, Milpark Hospital, Johannesburg, South Africa
  • Villegas, Maria-Virginia International Center for Medical Research and Training, CIDEIM, Cali, Colombia
  • Dubois, Véronique Laboratory of Bacteriology, Bordeaux University Hospital, Bordeaux, France - CNRS UMR5234, University of Bordeaux, France
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - INSERM European Unit (LEA Paris, IAME, France), University of Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance, University of Fribourg, Switzerland
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    11.01.2017
Published in:
  • Antimicrobial Agents and Chemotherapy. - 2017, vol. 61, no. 11, p. e01423-17
English Mutations in crrAB genes encoding a two-component regulator involved in modifications of lipopolysaccharide were searched for among a collection of colistin- resistant Klebsiella pneumoniae isolates. Four isolates, respectively, producing carbapenemases NDM-1, OXA-181, or KPC-2 showed mutated CrrB proteins compared with those in wild-type strains. Complementation assays with a wild-type CrrB protein restored the susceptibility to colistin in all cases, confirming the involvement of the identified substitutions in the resistance phenotype.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306106
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