Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

Alvarez-Marin, R.; Aires-de-Sousa, Marta; Nordmann, Patrice; Kieffer, Nicolas; Poirel, Laurent

doi:10.1007/s10096-017-3070-0

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Journal article

Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

Alvarez-Marin, R. Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland - Hospital Universitario Virgen del Rocio y Virgen Macarena, Seville, Spain
Aires-de-Sousa, Marta Escola Superior de Saúde da Cruz Vermelha Portuguesa, Lisbon, Portugal
Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland - University of Lausanne and University Hospital Center, Lausanne, Switzerland
Kieffer, Nicolas Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland
Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland

19.08.2017

Published in:

European Journal of Clinical Microbiology & Infectious Diseases. - 2017, p. 1–5

English Multidrug-resistant (MR) Gram-negative (GN) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. Well-tolerated antiseptics, such as octenidine dihydrochloride (OCT), may be a very useful tool in infection control to reduce the dissemination of MRGN. This study aimed to investigate the bactericidal activity of OCT against international epidemic clones of MRGN. A set of five different species (Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa) was studied to prove OCT efficacy without organic load, under “clean conditions” (0.3 g/L albumin) and under “dirty conditions” (3 g/L albumin + 3 mL/L defibrinated sheep blood), according to an official test norm (EN13727). We used five clonally unrelated isolates per species, including a susceptible wild-type strain, and four MRGN isolates, corresponding to either the 3MRGN or 4MRGN definition of multidrug resistance. A contact time of 1 min was fully effective for all isolates by using different OCT concentrations (0.01% and 0.05%), with a bacterial reduction factor of >5 log10 systematically observed. Growth kinetics were determined with two different wild-type strains (A. baumannii and K. pneumoniae), proving a time-dependent efficacy of OCT. These results highlight that OCT may be extremely useful to eradicate emerging highly resistant Gram-negative pathogens associated with nosocomial infections.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language