Journal article

Antimicrobial activity of octenidine against multidrug-resistant Gram-negative pathogens

  • Alvarez-Marin, R. Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland - Hospital Universitario Virgen del Rocio y Virgen Macarena, Seville, Spain
  • Aires-de-Sousa, Marta Escola Superior de Saúde da Cruz Vermelha Portuguesa, Lisbon, Portugal
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland - University of Lausanne and University Hospital Center, Lausanne, Switzerland
  • Kieffer, Nicolas Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - French INSERM European UnitUniversity of Fribourg (LEA-IAME), Fribourg, Switzerland - National Reference Center for Emerging Antibiotic Resistance (Switzerland), Fribourg, Switzerland
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    19.08.2017
Published in:
  • European Journal of Clinical Microbiology & Infectious Diseases. - 2017, p. 1–5
English Multidrug-resistant (MR) Gram-negative (GN) pathogens pose a major and growing threat for healthcare systems, as therapy of infections is often limited due to the lack of available systemic antibiotics. Well-tolerated antiseptics, such as octenidine dihydrochloride (OCT), may be a very useful tool in infection control to reduce the dissemination of MRGN. This study aimed to investigate the bactericidal activity of OCT against international epidemic clones of MRGN. A set of five different species (Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Acinetobacter baumannii, and Pseudomonas aeruginosa) was studied to prove OCT efficacy without organic load, under “clean conditions” (0.3 g/L albumin) and under “dirty conditions” (3 g/L albumin + 3 mL/L defibrinated sheep blood), according to an official test norm (EN13727). We used five clonally unrelated isolates per species, including a susceptible wild-type strain, and four MRGN isolates, corresponding to either the 3MRGN or 4MRGN definition of multidrug resistance. A contact time of 1 min was fully effective for all isolates by using different OCT concentrations (0.01% and 0.05%), with a bacterial reduction factor of >5 log10 systematically observed. Growth kinetics were determined with two different wild-type strains (A. baumannii and K. pneumoniae), proving a time-dependent efficacy of OCT. These results highlight that OCT may be extremely useful to eradicate emerging highly resistant Gram-negative pathogens associated with nosocomial infections.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/306066
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