Journal article

+ 1 other files

Stem cell factor-based identification and functional properties of in vitro-selected subpopulations of malignant mesothelioma cells

  • Blum, Walter Unit of Anatomy, Department of Medicine, University of Fribourg, Switzerland
  • Pecze, László Unit of Anatomy, Department of Medicine, University of Fribourg, Switzerland
  • Felley-Bosco, Emanuela Laboratory of Molecular Oncology, Lungen- und Thoraxonkologie Zentrum, University Hospital Zürich, Switzerland
  • Wu, Licun Latner Thoracic Surgery Research Laboratories, Division of Thoracic Surgery, Toronto General Hospital, University Health Network, University of Toronto, Canada
  • Perrot, Marc de Latner Thoracic Surgery Research Laboratories, Division of Thoracic Surgery, Toronto General Hospital, University Health Network, University of Toronto, Canada
  • Schwaller, Beat Unit of Anatomy, Department of Medicine, University of Fribourg, Switzerland
Show more…
    11.04.2017
Published in:
  • Stem Cell Reports. - 2017, vol. 8, no. 4, p. 1005–1017
English Malignant mesothelioma (MM) is an aggressive neoplasm characterized by a poor patient survival rate, because of rapid tumor recurrence following first-line therapy. Cancer stem cells (CSCs) are assumed to be responsible for initiating tumorigenesis and driving relapse after therapeutic interventions. CSC-enriched MM cell subpopulations were identified by an OCT4/SOX2 reporter approach and were characterized by (1) increased resistance to cisplatin, (2) increased sensitivity toward the FAK inhibitor VS-6063 in vitro, and (3) a higher tumor-initiating capacity in vivo in orthotopic xenograft and allograft mouse models. Overexpression of NF2 (neurofibromatosis 2, merlin), a tumor suppressor often mutated or lost in MM, did not affect proliferation and viability of CSC-enriched MM populations but robustly decreased the viability of reporter-negative cells. In contrast, downregulation of calretinin strongly decreased proliferation and viability of both populations. In summary, we have enriched and characterized a small MM cell subpopulation that bears the expected CSC characteristics.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305784
Other files

Statistics

Document views: 12 File downloads:
  • sch_scf.pdf: 2
  • sch_scf_sm1.pdf: 0