Journal article
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ER membrane phospholipids and surface tension control cellular lipid droplet formation
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M'barek, Kalthoum Ben
Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
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Ajjaji, Dalila
Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
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Chorlay, Aymeric
Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
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Vanni, Stefano
Department of Biology, University of Fribourg, Switzerland
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Forêt, Lionel
Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
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Thiam, Abdou Rachid
Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
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Published in:
- Developmental Cell. - 2017, vol. 41, no. 6, p. 591–604.e7
English
Cells convert excess energy into neutral lipids that are made in the endoplasmic reticulum (ER) bilayer. The lipids are then packaged into spherical or budded lipid droplets (LDs) covered by a phospholipid monolayer containing proteins. LDs play a key role in cellular energy metabolism and homeostasis. A key unanswered question in the life of LDs is how they bud off from the ER. Here, we tackle this question by studying the budding of artificial LDs from model membranes. We find that the bilayer phospholipid composition and surface tension are key parameters of LD budding. Phospholipids have differential LD budding aptitudes, and those inducing budding decrease the bilayer tension. We observe that decreasing tension favors the egress of neutral lipids from the bilayer and LD budding. In cells, budding conditions favor the formation of small LDs. Our discovery reveals the importance of altering ER physical chemistry for controlled cellular LD formation.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Biologie
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/305544
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