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ER membrane phospholipids and surface tension control cellular lipid droplet formation

  • M'barek, Kalthoum Ben Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
  • Ajjaji, Dalila Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
  • Chorlay, Aymeric Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
  • Vanni, Stefano Department of Biology, University of Fribourg, Switzerland
  • Forêt, Lionel Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
  • Thiam, Abdou Rachid Laboratoire de Physique Statistique, Département de Physique de l’ENS, Université Paris Diderot, Sorbonne Paris Cité, Paris, France
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    19.06.2017
Published in:
  • Developmental Cell. - 2017, vol. 41, no. 6, p. 591–604.e7
English Cells convert excess energy into neutral lipids that are made in the endoplasmic reticulum (ER) bilayer. The lipids are then packaged into spherical or budded lipid droplets (LDs) covered by a phospholipid monolayer containing proteins. LDs play a key role in cellular energy metabolism and homeostasis. A key unanswered question in the life of LDs is how they bud off from the ER. Here, we tackle this question by studying the budding of artificial LDs from model membranes. We find that the bilayer phospholipid composition and surface tension are key parameters of LD budding. Phospholipids have differential LD budding aptitudes, and those inducing budding decrease the bilayer tension. We observe that decreasing tension favors the egress of neutral lipids from the bilayer and LD budding. In cells, budding conditions favor the formation of small LDs. Our discovery reveals the importance of altering ER physical chemistry for controlled cellular LD formation.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305544
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