Journal article

Endogenous TRPV1 stimulation leads to the activation of the inositol phospholipid pathway necessary for sustained Ca2+ oscillations

  • Pecze, László Anatomy, Department of Medicine, University of Fribourg, Switzerland
  • Blum, Walter Anatomy, Department of Medicine, University of Fribourg, Switzerland
  • Henzi, Thomas Anatomy, Department of Medicine, University of Fribourg, Switzerland
  • Schwaller, Beat Anatomy, Department of Medicine, University of Fribourg, Switzerland
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    20.09.2016
Published in:
  • Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. - 2016, vol. 1863, no. 12, p. 2905–2915
English Sensory neuron subpopulations as well as breast and prostate cancer cells express functional transient receptor potential vanilloid type 1 (TRPV1) ion channels; however little is known how TRPV1 activation leads to biological responses. Agonist-induced activation of TRPV1 resulted in specific spatiotemporal patterns of cytoplasmic Ca²⁺ signals in breast and prostate cancer-derived cells. Capsaicin (CAPS; 50 μM) evoked intracellular Ca²⁺ oscillations and/or intercellular Ca²⁺ waves in all cell lines. As evidenced in prostate cancer Du 145 cells, oscillations were largely dependent on the expression of functional TRPV1 channels in the plasma membrane, phospholipase C activation and on the presence of extracellular Ca²⁺ ions. Concomitant oscillations of the mitochondrial matrix Ca²⁺ concentration resulted in mitochondria energization evidenced by increased ATP production. CAPS-induced Ca²⁺ oscillations also occurred in a subset of sensory neurons, yet already at lower CAPS concentrations (1 μM). Stimulation of ectopically expressed TRPV1 channels in CAPS-insensitive NIH- 3T3 cells didn't provoke CAPS-triggered Ca²⁺ oscillations; rather it resulted in low- magnitude, long-lasting elevations of the cytosolic Ca²⁺ concentration. This indicates that sole TRPV1 activation is not sufficient to generate Ca²⁺ oscillations. Instead the initial TRPV1-mediated signal leads to the activation of the inositol phospholipid pathway. This in turn suffices to generate a biologically relevant frequency-modulated Ca²⁺ signal.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305435
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