Functional mapping of yeast genomes by saturated transposition
- Michel, Agnès Henria Institute of Biochemistry, ETH Zurich, Switzerland
- Hatakeyama, Riko Department of Biology, University of Fribourg, Switzerland.
- Kimmig, Philipp Institute of Biochemistry, ETH Zurich, Switzerland
- Arter, Meret Institute of Biochemistry, ETH Zurich, Switzerland
- Peter, Matthias Institute of Biochemistry, ETH Zurich, Switzerland
- Matos, Joao Institute of Biochemistry, ETH Zurich, Switzerland
- De Virgilio, Claudio Department of Biology, University of Fribourg, Switzerland.
- Kornmann, Benoît Institute of Biochemistry, ETH Zurich, Switzerland
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08.05.2017
Published in:
- eLife. - 2017, vol. 6, p. e23570
English
Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposons can insert without disrupting essential functions. SATAY is particularly suited to discover loci important for growth under various conditions. SATAY (1) reveals positive and negative genetic interactions in single and multiple mutant strains, (2) can identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4) generates both null and other informative alleles. In a SATAY screen for rapamycin-resistant mutants, we identify Pib2 (PhosphoInositide-Binding 2) as a master regulator of TORC1. We describe two antagonistic TORC1-activating and -inhibiting activities located on opposite ends of Pib2. Thus, SATAY allows to easily explore the yeast genome at unprecedented resolution and throughput.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 288907
- DOI 10.7554/eLife.23570
- Persistent URL
- https://folia.unifr.ch/unifr/documents/305419
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