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Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity

  • Kuhn, Benjamin M. Institute of Plant and Microbial Biology, University of Zurich, Switzerland
  • Nodzyński, Tomasz Mendel Centre for Plant Genomics and Proteomics, CEITEC, Masaryk University, Brno, Czech Republic
  • Errafi, Sanae Institute of Plant and Microbial Biology, University of Zurich, Switzerland
  • Bucher, Rahel Institute of Chemistry, University of Zurich, Switzerland
  • Gupta, Shibu Institute of Plant and Microbial Biology, University of Zurich, Switzerland
  • Aryal, Bibek Department of Biology –geislerLab, University of Fribourg, Switzerland
  • Dobrev, Petre Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Bigler, Laurent Institute of Chemistry, University of Zurich, Switzerland
  • Geisler, Markus Department of Biology –geislerLab, University of Fribourg, Switzerland
  • Zažímalová, Eva Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
  • Friml, Jiří Institute of Science and Technology Austria (IST Austria), Klosterneuburg, Austria
  • Ringli, Christoph Institute of Plant and Microbial Biology, University of Zurich, Switzerland
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    06.02.2017
Published in:
  • Scientific Reports. - 2017, vol. 7, p. 41906
English The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Flavonols have been shown to alter auxin transport activity and changes in flavonol accumulation in the Arabidopsis thaliana rol1-2 mutant cause defects in auxin transport and seedling development. A new mutation in ROOTS CURL IN NPA 1 (RCN1), encoding a regulatory subunit of the phosphatase PP2A, was found to suppress the growth defects of rol1-2 without changing the flavonol content. rol1-2 rcn1-3 double mutants show wild type-like auxin transport activity while levels of free auxin are not affected by rcn1-3. In the rol1-2 mutant, PIN2 shows a flavonol-induced basal-to-apical shift in polar localization which is reversed in the rol1-2 rcn1-3 to basal localization. In vivo analysis of PINOID action, a kinase known to influence PIN protein localization in a PP2A-antagonistic manner, revealed a negative impact of flavonols on PINOID activity. Together, these data suggest that flavonols affect auxin transport by modifying the antagonistic kinase/phosphatase equilibrium.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305392
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