Journal article

Pavlovian conditioning of larval drosophila: an illustrated, multilingual, hands-on manual for odor-taste associative learning in maggots

  • Michels, Birgit Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • Saumweber, Timo Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • Biernacki, Roland Department Neurobiology and Genetics, Julius Maximilians University, Würzburg, Germany
  • Thum, Jeanette Department Neurobiology and Genetics, Julius Maximilians University, Würzburg, Germany
  • Glasgow, Rupert D. V. Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • Schleyer, Michael Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • Chen, Yi-chun Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • Eschbach, Claire HHMI Janelia Research Campus, Ashburn, VA, USA
  • Stocker, Reinhard F. Department of Biology, Université de Fribourg, Switzerland
  • Toshima, Naoko Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
  • Tanimura, Teiichi Department of Biology, Kyushu University, Fukuoka, Japan
  • Louis, Matthieu Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, USA
  • Arias-Gil, Gonzalo Department Systems Physiology, Leibniz Institute for Neurobiology Magdeburg, Germany
  • Marescotti, Manuela Brainwave Discovery Ltd., Edinburgh, UK
  • Benfenati, Fabio Italian Institute of Technology, Center for Synaptic Neuroscience and Technology, Genova, Italy
  • Gerber, Bertram Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany - Institute of Biology, Otto von Guericke University, Magdeburg, Germany - Center for Behavioral Brain Sciences, Magdeburg, Germany
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    2017
Published in:
  • Frontiers in Behavioral Neuroscience / Published version ; http://dx.doi.org/10.3389/fnbeh.2017.00045. - 2017, vol. 11, p. 45
English Larval Drosophila offer a study case for behavioral neurogenetics that is simple enough to be experimentally tractable, yet complex enough to be worth the effort. We provide a detailed, hands-on manual for Pavlovian odor-reward learning in these animals. Given the versatility of Drosophila for genetic analyses, combined with the evolutionarily shared genetic heritage with humans, the paradigm has utility not only in behavioral neurogenetics and experimental psychology, but for translational biomedicine as well. Together with the upcoming total synaptic connectome of the Drosophila nervous system and the possibilities of single-cell-specific transgene expression, it offers enticing opportunities for research. Indeed, the paradigm has already been adopted by a number of labs and is robust enough to be used for teaching in classroom settings. This has given rise to a demand for a detailed, hands- on manual directed at newcomers and/or at laboratory novices, and this is what we here provide.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305384
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