Pavlovian conditioning of larval drosophila: an illustrated, multilingual, hands-on manual for odor-taste associative learning in maggots

Michels, Birgit; Saumweber, Timo; Biernacki, Roland; Thum, Jeanette; Glasgow, Rupert D. V.; Schleyer, Michael; Chen, Yi-chun; Eschbach, Claire; Stocker, Reinhard F.; Toshima, Naoko; Tanimura, Teiichi; Louis, Matthieu; Arias-Gil, Gonzalo; Marescotti, Manuela; Benfenati, Fabio; Gerber, Bertram

doi:10.3389/fnbeh.2017.00045

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Journal article

Pavlovian conditioning of larval drosophila: an illustrated, multilingual, hands-on manual for odor-taste associative learning in maggots

Michels, Birgit Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
Saumweber, Timo Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
Biernacki, Roland Department Neurobiology and Genetics, Julius Maximilians University, Würzburg, Germany
Thum, Jeanette Department Neurobiology and Genetics, Julius Maximilians University, Würzburg, Germany
Glasgow, Rupert D. V. Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
Schleyer, Michael Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
Chen, Yi-chun Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
Eschbach, Claire HHMI Janelia Research Campus, Ashburn, VA, USA
Stocker, Reinhard F. Department of Biology, Université de Fribourg, Switzerland
Toshima, Naoko Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany
Tanimura, Teiichi Department of Biology, Kyushu University, Fukuoka, Japan
Louis, Matthieu Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, USA
Arias-Gil, Gonzalo Department Systems Physiology, Leibniz Institute for Neurobiology Magdeburg, Germany
Marescotti, Manuela Brainwave Discovery Ltd., Edinburgh, UK
Benfenati, Fabio Italian Institute of Technology, Center for Synaptic Neuroscience and Technology, Genova, Italy
Gerber, Bertram Department of Genetics, Leibniz Institute for Neurobiology, Magdeburg, Germany - Institute of Biology, Otto von Guericke University, Magdeburg, Germany - Center for Behavioral Brain Sciences, Magdeburg, Germany

2017

Published in:

Frontiers in Behavioral Neuroscience / Published version ; http://dx.doi.org/10.3389/fnbeh.2017.00045. - 2017, vol. 11, p. 45

English Larval Drosophila offer a study case for behavioral neurogenetics that is simple enough to be experimentally tractable, yet complex enough to be worth the effort. We provide a detailed, hands-on manual for Pavlovian odor-reward learning in these animals. Given the versatility of Drosophila for genetic analyses, combined with the evolutionarily shared genetic heritage with humans, the paradigm has utility not only in behavioral neurogenetics and experimental psychology, but for translational biomedicine as well. Together with the upcoming total synaptic connectome of the Drosophila nervous system and the possibilities of single-cell-specific transgene expression, it offers enticing opportunities for research. Indeed, the paradigm has already been adopted by a number of labs and is robust enough to be used for teaching in classroom settings. This has given rise to a demand for a detailed, hands- on manual directed at newcomers and/or at laboratory novices, and this is what we here provide.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language