Two- and three-dimensional mid-infrared chemical imaging
Zobi, FabioDepartment of Chemistry, University of Fribourg, Switzerland
Obst, MartinBayCEER, University of Bayreuth, Germany
22.02.2017
Published in:
CHIMIA International Journal for Chemistry. - 2017, vol. 71, no. 1, p. 32–37
English
This article describes the state-of-the-art in 2D and 3D tomographic mid-IR imaging and its current and potential applications in the imaging of material and biological systems with a focus on cells and tissues. 2D FTIR microscopy is first presented in terms of the basic experimental configurations of the technique, optics, data and image acquisition. This first section provides a basis for the ensuing discussion on 3D FTIR spectro-microtomography. In the second part, examples of 2D and 3D chemical imaging are given with a focus on the use of molecular chemical probes and probe-free applications, respectively.