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Vitamin C promotes pluripotency of human induced pluripotent stem cells via the histone demethylase JARID1A

  • Eid, Wassim Biochemistry Department, Medical Research Institute, University of Alexandria, Egypt - Division of Endocrinology, Department of Medicine, University of Fribourg, Switzerland
  • Abdel-Rehim, Wafaa Biochemistry Department, Medical Research Institute, University of Alexandria, Egypt
    2016
Published in:
  • Biological Chemistry. - 2016, vol. 397, no. 11, p. 1205-1213
English Somatic cells can be reprogramed into induced pluripotent stem (iPS) cells by defined factors, which provide a powerful basis for personalized stem-cell based therapies. However, cellular reprograming is an inefficient and metabolically demanding process commonly associated with obstacles that hamper further use of this technology. Spontaneous differentiation of iPS cells cultures represents a significant hurdle that hinder obtaining high quality iPS cells for further downstream experimentation. In this study, we found that a natural compound, vitamin C, augmented pluripotency in iPS cells and reduced unwanted spontaneous differentiation during iPS cells maintenance. Gene expression analysis showed that vitamin C increased the expression of the histone demethylase JARID1A. Furthermore, through gain- and loss-of-function approaches, we show that JARID1A is a key effector in promoting pluripotency and reducing differentiation downstream of vitamin C. Our results therefore highlight a straightforward method for improving the pluripotency and quality of iPS cells; it also shows a possible role for H3K4me2/3 in cell fate determination and establishes a link between vitamin C and epigenetic regulation.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biological sciences
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/305352
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