Journal article

Rapid Aminoglycoside NP test for rapid detection of multiple aminoglycoside resistance in Enterobacteriaceae

  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France - INSERM European Unit (LEA, IAME, Paris), Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Institute of Microbiology, University Hospital Centre and University of Lausanne, Switzerland
  • Jayol, Aurélie Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France - INSERM European Unit (LEA, IAME, Paris), Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Laboratoire de Bactériologie, CHU de Bordeaux, France - CNRS UMR5234 Microbiologie Fondamentale et Pathogénicité, University of Bordeaux, France
  • Dobias, Jan Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, France
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    18.01.2017
Published in:
  • Journal of Clinical Microbiology. - 2017, p. JCM.02107-16
English The Rapid Aminoglycoside NP (Nordmann/Poirel) test was developed to rapidly identify multiple aminoglycoside (AG) resistance in Enterobacteriaceae. It is based on the detection of the glucose metabolism related to enterobacterial growth in presence of a defined concentration of amikacin plus gentamicin. Formation of acid metabolites was evidenced by a color change (orange to yellow) of the red phenol pH indicator. The Rapid Aminoglycoside NP test was evaluated by using bacterial colonies of 18 AG- resistant isolates producing 16S rRNA methylases, 20 AG-resistant isolates expressing AG-modifying enzymes (acetyl, adenyl and phospho transferases), and 10 isolates susceptible to AG. Its sensitivity and specificity were 100% and 97% respectively, as compared to the broth dilution method taken as the gold standard for determining aminoglycoside resistance. The test is inexpensive, rapid (<2 hours), and implementable worldwide.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305303
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