Protein phosphatase AP2C1 negatively regulates basal resistance and defense responses to Pseudomonas syringae

Shubchynskyy, Volodymyr; Boniecka, Justyna; Schweighofer, Alois; Simulis, Justinas; Kvederaviciute, Kotryna; Stumpe, Michael; Mauch, Felix; Balazadeh, Salma; Mueller-Roeber, Bernd; Boutrot, Freddy; Zipfel, Cyril; Meskiene, Irute

doi:10.1093/jxb/erw485

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Protein phosphatase AP2C1 negatively regulates basal resistance and defense responses to Pseudomonas syringae

Shubchynskyy, Volodymyr Max F. Perutz Laboratories, University and Medical University of Vienna, Austria
Boniecka, Justyna Max F. Perutz Laboratories, University and Medical University of Vienna, Austria
Schweighofer, Alois Max F. Perutz Laboratories, University and Medical University of Vienna, Austria - Institute of Biotechnology (IBT), University of Vilnius, Lithuania
Simulis, Justinas Institute of Biotechnology (IBT), University of Vilnius, Lithuania
Kvederaviciute, Kotryna Institute of Biotechnology (IBT), University of Vilnius, Lithuania
Stumpe, Michael Department of Biology, University of Fribourg, Switzerland
Mauch, Felix Department of Biology, University of Fribourg, Switzerland
Balazadeh, Salma Max-Planck-Institute for Molecular Plant Physiology, Golm and University of Potsdam, Germany
Mueller-Roeber, Bernd Max-Planck-Institute for Molecular Plant Physiology, Golm and University of Potsdam, Germany
Boutrot, Freddy The Sainsbury Laboratory, Norwich Research Park, Norwich UK
Zipfel, Cyril The Sainsbury Laboratory, Norwich Research Park, Norwich UK
Meskiene, Irute Max F. Perutz Laboratories, University and Medical University of Vienna, Austria - Institute of Biotechnology (IBT), University of Vilnius, Lithuania - Department of Ecogenomics and Systems Biology, University of Vienna, Austria

2017

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Journal of Experimental Botany. - 2017, vol. 68, no. 5, p. 1169-1183

English Mitogen-activated protein kinases (MAPKs) mediate plant immune responses to pathogenic bacteria. However, less is known about the cell autonomous negative regulatory mechanism controlling basal plant immunity. We report the biological role of Arabidopsis thaliana MAPK phosphatase AP2C1 as a negative regulator of plant basal resistance and defense responses to Pseudomonas syringae. AP2C2, a closely related MAPK phosphatase, also negatively controls plant resistance. Loss of AP2C1 leads to enhanced pathogen-induced MAPK activities, increased callose deposition in response to pathogen-associated molecular patterns or to P. syringae pv. tomato (Pto) DC3000, and enhanced resistance to bacterial infection with Pto. We also reveal the impact of AP2C1 on the global transcriptional reprogramming of transcription factors during Pto infection. Importantly, ap2c1 plants show salicylic acid-independent transcriptional reprogramming of several defense genes and enhanced ethylene production in response to Pto. This study pinpoints the specificity of MAPK regulation by the different MAPK phosphatases AP2C1 and MKP1, which control the same MAPK substrates, nevertheless leading to different downstream events. We suggest that precise and specific control of defined MAPKs by MAPK phosphatases during plant challenge with pathogenic bacteria can strongly influence plant resistance.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language