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Combined exposure of diesel exhaust particles and respirable Soufrière Hills volcanic ash causes a (pro-)inflammatory response in an in vitro multicellular epithelial tissue barrier model

  • Tomašek, Ines Institute of Hazard, Risk and Resilience, Department of Earth Sciences, Durham University, UK - BioNanomaterials,Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Horwell, Claire J. Institute of Hazard, Risk and Resilience, Department of Earth Sciences, Durham University, UK
  • Damby, David E. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Germany - United States Geological Survey, Menlo Park, CA USA
  • Barošová, Hana BioNanomaterials,Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Geers, Christoph BioNanomaterials,Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Petri-Fink, Alke BioNanomaterials,Adolphe Merkle Institute, University of Fribourg, Switzerland - Chemistry Department, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara BioNanomaterials,Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Clift, Martin J. D. BioNanomaterials,Adolphe Merkle Institute, University of Fribourg, Switzerland - In Vitro Toxicology Group, Institute of Life Sciences, Swansea University Medical School, Wales, UK
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    2016
Published in:
  • Particle and Fibre Toxicology. - 2016, vol. 13, p. 67
Volcanic ash
Diesel exhaust particles
In vitro
Particle co-exposures
Multicellular Human Epithelial Tissue Barrier
System Air-liquid Interface Exposures
(pro-)inflammatory cytokines/chemokines
English There are justifiable health concerns regarding the potential adverse effects associated with human exposure to volcanic ash (VA) particles, especially when considering communities living in urban areas already exposed to heightened air pollution. The aim of this study was, therefore, to gain an imperative, first understanding of the biological impacts of respirable VA when exposed concomitantly with diesel particles.Methods: A sophisticated in vitro 3D triple cell co-culture model of the human alveolar epithelial tissue barrier was exposed to either a single or repeated dose of dry respirable VA (deposited dose of 0.26 ± 0.09 or 0.89 ± 0.29 μg/cm2, respectively) from Soufrière Hills volcano, Montserrat for a period of 24 h at the air- liquid interface (ALI). Subsequently, co-cultures were exposed to co-exposures of single or repeated VA and diesel exhaust particles (DEP; NIST SRM 2975; 0.02 mg/mL), a model urban pollutant, at the pseudo-ALI. The biological impact of each individual particle type was also analysed under these precise scenarios. The cytotoxic (LDH release), oxidative stress (depletion of intracellular GSH) and (pro-)inflammatory (TNF-α, IL-8 and IL-1β) responses were assessed after the particulate exposures. The impact of VA exposure upon cell morphology, as well as its interaction with the multicellular model, was visualised via confocal laser scanning microscopy (LSM) and scanning electron microscopy (SEM), respectively.Results: The combination of respirable VA and DEP, in all scenarios, incited an heightened release of TNF-α and IL-8 as well as significant increases in IL-1β, when applied at sub-lethal doses to the co-culture compared to VA exposure alone. Notably, the augmented (pro-)inflammatory responses observed were not mediated by oxidative stress. LSM supported the quantitative assessment of cytotoxicity, with no changes in cell morphology within the barrier model evident. A direct interaction of the VA with all three cell types of the multicellular system was observed by SEM.Conclusions: Combined exposure of respirable Soufrière Hills VA with DEP causes a (pro-)inflammatory effect in an advanced in vitro multicellular model of the epithelial airway barrier. This finding suggests that the combined exposure to volcanic and urban particulate matter should be further investigated in order to deduce the potential human health hazard, especially how it may influence the respiratory function of susceptible individuals (i.e. with pre-existing lung diseases) in the population.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305245
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