Journal article

Parvalbumin-neurons of the ventrolateral hypothalamic parvafox nucleus receive a glycinergic input: a gene-microarray study

  • Szabolcsi, Viktoria Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg, Switzerland
  • Albisetti, Gioele W. Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg, Switzerland
  • Celio, Marco R. Anatomy and Program in Neuroscience, Department of Medicine, University of Fribourg, Switzerland
    2017
Published in:
  • Frontiers in Molecular Neuroscience. - 2017, vol. 10, p. 8
English The ventrolateral hypothalamic parvafox (formerly called PV1-Foxb1) nucleus is an anatomical entity of recent discovery and unknown function. With a view to gaining an insight into its putative functional role(s), we conducted a gene-microarray analysis and, armed with the forthcoming data, controlled the results with the Allen databases and the murine BrainStars (B*) database. The parvafox nucleus was specifically sampled by laser-capture microdissection and the transcriptome was subjected to a microarray analysis on Affymetrix chips. Eighty-two relevant genes were found to be potentially more expressed in this brain region than in either the cerebral cortex or the hippocampus. When the expression patterns of these genes were counterchecked in the Allen-Database of in-situ hybridizations and in the B*-microarray database, their localization in the parvafox region was confirmed for thirteen. For nine novel genes, which are particularly interesting because of their possible involvement in neuromodulation, the expression was verified by quantitative real time-PCR. Of particular functional importance may be the occurrence of glycine receptors, the presence of which indicates that the activity of the parvafox nucleus is under ascending inhibitory control.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305244
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