Journal article

REV-ERBα influences the stability and nuclear localization of the glucocorticoid receptor

  • Okabe, Takashi Dept. of Biology, Biochemistry, University of Fribourg, Switzerland
  • Chavan, Rohit Dept. of Biology, Biochemistry, University of Fribourg, Switzerland
  • Costa, Sara S. Fonseca Dept. of Biology, Biochemistry, University of Fribourg, Switzerland
  • Brenna, Andrea Dept. of Biology, Biochemistry, University of Fribourg, Switzerland
  • Ripperger, Jürgen A. Dept. of Biology, Biochemistry, University of Fribourg, Switzerland
  • Albrecht, Urs Dept. of Biology, Biochemistry, University of Fribourg, Switzerland
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    01.11.2016
Published in:
  • Journal of Cell Science. - 2016, vol. 129, no. 21, p. 4143–4154
English REV-ERBα (encoded by Nr1d1) is a nuclear receptor that is part of the circadian clock mechanism and regulates metabolism and inflammatory processes. The glucocorticoid receptor (GR, encoded by Nr3c1) influences similar processes, but is not part of the circadian clock, although glucocorticoid signaling affects resetting of the circadian clock in peripheral tissues. Because of their similar impact on physiological processes, we studied the interplay between these two nuclear receptors. We found that REV- ERBα binds to the C-terminal portion and GR to the N-terminal portion of HSP90α and HSP90β, a chaperone responsible for the activation of proteins to ensure survival of a cell. The presence of REV-ERBα influences the stability and nuclear localization of GR by an unknown mechanism, thereby affecting expression of GR target genes, such as IκBα (Nfkbia) and alcohol dehydrogenase 1 (Adh1). Our findings highlight an important interplay between two nuclear receptors that influence the transcriptional potential of each other. This indicates that the transcriptional landscape is strongly dependent on dynamic processes at the protein level.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie, Département de Médecine
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305235
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