Journal article

+ 1 other files

Mature lipid droplets are accessible to ER luminal proteins

  • Mishra, Shirish University of Fribourg, Department of Biology, Fribourg, Switzerland
  • Khaddaj, Rasha University of Fribourg, Department of Biology, Fribourg, Switzerland
  • Cottier, Stéphanie University of Fribourg, Department of Biology, Fribourg, Switzerland
  • Stradalova, Vendula University of Fribourg, Department of Biology, Fribourg, Switzerland
  • Jacob, Claire University of Fribourg, Department of Biology, Fribourg, Switzerland
  • Schneiter, Roger University of Fribourg, Department of Biology, Fribourg, Switzerland
Show more…
    15.10.2016
Published in:
  • Journal of Cell Science. - 2016, vol. 129, no. 20, p. 3803–3815
English Lipid droplets are found in most organisms where they serve to store energy in the form of neutral lipids. They are formed at the endoplasmic reticulum (ER) membrane where the neutral-lipid-synthesizing enzymes are located. Recent results indicate that lipid droplets remain functionally connected to the ER membrane in yeast and mammalian cells to allow the exchange of both lipids and integral membrane proteins between the two compartments. The precise nature of the interface between the ER membrane and lipid droplets, however, is still ill-defined. Here, we probe the topology of lipid droplet biogenesis by artificially targeting proteins that have high affinity for lipid droplets to inside the luminal compartment of the ER. Unexpectedly, these proteins still localize to lipid droplets in both yeast and mammalian cells, indicating that lipid droplets are accessible from within the ER lumen. These data are consistent with a model in which lipid droplets form a specialized domain in the ER membrane that is accessible from both the cytosolic and the ER luminal side.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305205
Other files

Statistics

Document views: 24 File downloads:
  • sch_mld.pdf: 42
  • sch_mld_sm.pdf: 19