Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

Girlich, Delphine; Dortet, Laurent; Poirel, Laurent; Nordmann, Patrice

doi:10.1093/jac/dku371

Back

Journal article

+ 1 other files

Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

Girlich, Delphine INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
Dortet, Laurent INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
Poirel, Laurent INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
Nordmann, Patrice INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland

01.01.2015

Published in:

Journal of Antimicrobial Chemotherapy. - 2015, vol. 70, no. 1, p. 98-102

English To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate.Methods: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing.Results: Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1- PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility.Conclusions: This is the first known description of the blaNDM-1 gene in a genomic island structure, which might further enhance the spread of the blaNDM-1 carbapenemase gene among enteric pathogens.

Faculty

Faculté des sciences et de médecine

Department

Médecine 3ème année

Language