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Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate

  • Girlich, Delphine INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
  • Dortet, Laurent INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
  • Poirel, Laurent INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
  • Nordmann, Patrice INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
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    01.01.2015
Published in:
  • Journal of Antimicrobial Chemotherapy. - 2015, vol. 70, no. 1, p. 98-102
English To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate.Methods: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing.Results: Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1- PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility.Conclusions: This is the first known description of the blaNDM-1 gene in a genomic island structure, which might further enhance the spread of the blaNDM-1 carbapenemase gene among enteric pathogens.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/305023
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