Integration of the blaNDM-1 carbapenemase gene into Proteus genomic island 1 (PGI1-PmPEL) in a Proteus mirabilis clinical isolate
- Girlich, Delphine INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
- Dortet, Laurent INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France
- Poirel, Laurent INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
- Nordmann, Patrice INSERM U914 ‘Emerging Resistance to Antibiotics’, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, University of Fribourg, Switzerland
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01.01.2015
Published in:
- Journal of Antimicrobial Chemotherapy. - 2015, vol. 70, no. 1, p. 98-102
English
To decipher the mechanisms and their associated genetic determinants responsible for β-lactam resistance in a Proteus mirabilis clinical isolate.Methods: The entire genetic structure surrounding the β-lactam resistance genes was characterized by PCR, gene walking and DNA sequencing.Results: Genes encoding the carbapenemase NDM-1 and the ESBL VEB-6 were located in a 38.5 kb MDR structure, which itself was inserted into a new variant of the Proteus genomic island 1 (PGI1). This new PGI1- PmPEL variant of 64.4 kb was chromosomally located, as an external circular form in the P. mirabilis isolate, suggesting potential mobility.Conclusions: This is the first known description of the blaNDM-1 gene in a genomic island structure, which might further enhance the spread of the blaNDM-1 carbapenemase gene among enteric pathogens.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 259492
- DOI 10.1093/jac/dku371
- Persistent URL
- https://folia.unifr.ch/unifr/documents/305023
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