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Functional genomics identifies regulators of the phototransduction machinery in the Drosophila larval eye and adult ocelli

  • Mishra, Abhishek Kumar Institute of Cell and Developmental Biology, Department of Biology, University of Fribourg, Switzerland
  • Bargmann, Bastiaan O.R. Cibus US LLC, San Diego, USA
  • Tsachaki, Maria Institute of Cell and Developmental Biology, Department of Biology, University of Fribourg, Switzerland
  • Fritsch, Cornelia Institute of Cell and Developmental Biology, Department of Biology, University of Fribourg, Switzerland
  • Sprecher, Simon G. Institute of Cell and Developmental Biology, Department of Biology, University of Fribourg, Switzerland
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    15.02.2016
Published in:
  • Developmental Biology. - 2016, vol. 410, no. 2, p. 164–177
drosophila
photoreceptor
larval eye
adult ocelli
rhodopsin
photoreceptor differentiation
phototransduction
English Sensory perception of light is mediated by specialized Photoreceptor neurons (PRs) in the eye. During development all PRs are genetically determined to express a specific Rhodopsin (Rh) gene and genes mediating a functional phototransduction pathway. While the genetic and molecular mechanisms of PR development is well described in the adult compound eye, it remains unclear how the expression of Rhodopsins and the phototransduction cascade is regulated in other visual organs in Drosophila, such as the larval eye and adult ocelli. Using transcriptome analysis of larval PR-subtypes and ocellar PRs we identify and study new regulators required during PR differentiation or necessary for the expression of specific signaling molecules of the functional phototransduction pathway. We found that the transcription factor Krüppel (Kr) is enriched in the larval eye and controls PR differentiation by promoting Rh5 and Rh6 expression. We also identified Camta, Lola, Dve and Hazy as key genes acting during ocellar PR differentiation. Further we show that these transcriptional regulators control gene expression of the phototransduction cascade in both larval eye and adult ocelli. Our results show that PR cell type-specific transcriptome profiling is a powerful tool to identify key transcriptional regulators involved during several aspects of PR development and differentiation. Our findings greatly contribute to the understanding of how combinatorial action of key transcriptional regulators control PR development and the regulation of a functional phototransduction pathway in both larval eye and adult ocelli.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/304895
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