7-rhamnosylated flavonols modulate homeostasis of the plant hormone auxin and affect plant development

Kuhn, Benjamin M.; Errafi, Sanae; Bucher, Rahel; Dobrev, Petre; Geisler, Markus; Bigler, Laurent; Zažímalová, Eva; Ringli, Christoph

doi:10.1074/jbc.M115.701565

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Journal article

7-rhamnosylated flavonols modulate homeostasis of the plant hormone auxin and affect plant development

Kuhn, Benjamin M. Department of Plant and Microbial Biology, University of Zurich, Switzerland
Errafi, Sanae Department of Plant and Microbial Biology, University of Zurich, Switzerland
Bucher, Rahel Department of Chemistry, University of Zurich, Switzerland
Dobrev, Petre Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Geisler, Markus Department of Biology, University of Fribourg, Switzerland
Bigler, Laurent Department of Chemistry, University of Zurich, Switzerland
Zažímalová, Eva Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic
Ringli, Christoph Department of Plant and Microbial Biology, University of Zurich, Switzerland

03.04.2016

Published in:

Journal of Biological Chemistry. - 2016, vol. 291, no. 10, p. 5385–5395

English Flavonols are a group of secondary metabolites that affect diverse cellular processes. They are considered putative negative regulators of the transport of the phytohormone auxin, by which they influence auxin distribution and concomitantly take part in the control of plant organ development. Flavonols are accumulating in a large number of glycosidic forms. Whether these have distinct functions and diverse cellular targets is not well understood. The rol1-2 mutant of Arabidopsis thaliana is characterized by a modified flavonol glycosylation profile that is inducing changes in auxin transport and growth defects in shoot tissues. To determine whether specific flavonol glycosides are responsible for these phenotypes, a suppressor screen was performed on the rol1-2 mutant, resulting in the identification of an allelic series of UGT89C1, a gene encoding a flavonol 7-O-rhamnosyltransferase. A detailed analysis revealed that interfering with flavonol rhamnosylation increases the concentration of auxin precursors and auxin metabolites, whereas auxin transport is not affected. This finding provides an additional level of complexity to the possible ways by which flavonols influence auxin distribution and suggests that flavonol glycosides play an important role in regulating plant development.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language