Journal article

Uninephrectomy-induced lipolysis and low-grade inflammation are mimicked by unilateral renal denervation

  • Arsenijevic, Denis Division of Physiology, Department of Medicine, University of Fribourg, Switzerland - National Center of Competence in Research (Kidney.CH), Zurich, Switzerland
  • Cajot, Jean-François Division of Physiology, Department of Medicine, University of Fribourg, Switzerland
  • Fellay, Benoit Chemistry/Hematology Laboratory, Fribourg Hospital, Switzerland
  • Dulloo, Abdul G. Division of Physiology, Department of Medicine, University of Fribourg, Switzerland
  • Vliet, Bruce N. Van BioMedical Sciences Division, Faculty of Medicine, Memorial University, St. John's, NL, Canada
  • Montani, Jean-Pierre Division of Physiology, Department of Medicine, University of Fribourg, Switzerland - National Center of Competence in Research (Kidney.CH), Zurich, Switzerland
Show more…
    2016
Published in:
  • Integrative Physiology. - 2016, p. 227
English Uninephrectomy (UniNX) in rats on a fixed food intake leads to increased lipolysis and a low-grade inflammation with an increased subset of circulating cytokines. Because UniNX ablates renal nerves on the side of the removed kidney, we tested the contribution of unilateral renal denervation in the phenotype of UniNX. We compared Sham-operated controls, left nephrectomy (UniNX) and unilateral left kidney denervation (uDNX) in rats 4 weeks after surgery. uDNX did not affect kidney weight and function. In general, the uDNX phenotype was similar to the UniNX phenotype especially for lipolysis in fat pads and increased low-grade inflammation. uDNX led to decreased fat pad weight and increased hormone sensitive lipase and adipocyte triglyceride lipase mRNA levels in epididymal and inguinal adipose tissue, as well as increased circulating lipolysis markers β-hydroxybutyrate and glycerol. Measured circulating hormones such as leptin, T3 and insulin were similar amongst the three groups. The lipolytic cytokines interferon-gamma and granulocyte macrophage colony stimulating factor were increased in the circulation of both uDNX and UniNX groups. These two cytokines were also elevated in the spleen of both groups, but contrastingly they were decreased in fat pads, liver, and kidneys. Both uDNX and UniNX similarly increased noradrenaline content in fat pads and spleen. Melanocortin 4 receptor mRNA levels were increased in the brains of both uDNX and UniNX compared to Sham and may contribute to increased tissue noradrenaline levels. In addition, the farnesoid x receptor (FXR) may contribute to changes in tissue metabolism and inflammation, as anti-inflammatory FXR was decreased in the spleen but increased in other tissues in uDNX and UniNX compared to Sham. In summary, both uDNX and UniNX in rats promote metabolic and immunological alterations by mechanisms that seem to implicate modification of unilateral renal nerve pathways as well as central and peripheral neural pathways.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304853
Statistics

Document views: 10 File downloads:
  • mon_uil.pdf: 2