Real-time PCR for detection of plasmid-mediated polymyxin resistance (mcr-1) from cultured bacteria and stools
- Bontron, Séverine Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland
- Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland
- Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - Hôpital Cantonal HFR, Fribourg, Switzerland
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27.04.2016
Published in:
- Journal of Antimicrobial Chemotherapy. - 2016, p. dkw139
English
The aim of the study was to develop a simple assay for rapid detection of the mcr-1 gene, recently identified as a source of plasmid-mediated acquired resistance to polymyxins in Enterobacteriaceae.Methods: A SYBR Green-based real-time PCR assay was designed for detection of the mcr-1 gene. This assay was applied to cultured bacteria and to spiked human and cattle stools.Results: The mcr-1 gene could be detected with a lower limit of 102 cultured bacteria. This test was highly sensitive and specific, and generated no false-positive results. The assay was also conclusive when applied to stools spiked with mcr-1-positive Escherichia coli.Conclusions: This simple, rapid, sensitive and specific assay will be useful for rapid screening of this resistance trait in both human medicine and veterinary medicine.
- Faculty
- Faculté des sciences et de médecine
- Department
- Médecine 3ème année
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 259510
- DOI 10.1093/jac/dkw139
- Persistent URL
- https://folia.unifr.ch/unifr/documents/304835
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