Journal article

Ultrathin ceramic membranes as scaffolds for functional cell coculture models on a biomimetic scale

  • Jud, Corinne Adolphe Merkle Institute, University of Fribourg, Switzerland - Agroscope, Institute for Livestock Sciences ILS, Posieux, Switzerland
  • Ahmed, Sher CSEM SA, Neuchâtel, Switzerland
  • Müller, Loretta University Children's Hospital Basel, Switzerland
  • Kinnear, Calum Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Umehara, Yuki Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Frey, Sabine Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Liley, Martha CSEM SA, Neuchâtel, Switzerland
  • Angeloni, Silvia CSEM SA, Neuchâtel, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
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    01.12.2015
Published in:
  • BioResearch Open Access. - 2015, vol. 4, no. 1, p. 457–468
English Epithelial tissue serves as an interface between biological compartments. Many in vitro epithelial cell models have been developed as an alternative to animal experiments to answer a range of research questions. These in vitro models are grown on permeable two-chamber systems; however, commercially available, polymer-based cell culture inserts are around 10 μm thick. Since the basement membrane found in biological systems is usually less than 1 μm thick, the 10-fold thickness of cell culture inserts is a major limitation in the establishment of realistic models. In this work, an alternative insert, accommodating an ultrathin ceramic membrane with a thickness of only 500 nm (i.e., the Silicon nitride Microporous Permeable Insert [SIMPLI]-well), was produced and used to refine an established human alveolar barrier coculture model by both replacing the conventional inserts with the SIMPLI-well and completing it with endothelial cells. The structural–functional relationship of the model was evaluated, including the translocation of gold nanoparticles across the barrier, revealing a higher translocation if compared to corresponding polyethylene terephthalate (PET) membranes. This study demonstrates the power of the SIMPLI-well system as a scaffold for epithelial tissue cell models on a truly biomimetic scale, allowing construction of more functionally accurate models of human biological barriers.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304771
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