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The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen

  • Ritpitakphong, Unyarat Department of Biology, University of Fribourg, Switzerland
  • Falquet, Laurent Department of Biology, University of Fribourg, Switzerland - Swiss Institute of Bioinformatics, University of Fribourg, Switzerland
  • Vimoltust, Artit Product & Technology Development Center, SCG Paper Plc, Bangsue, Bangkok, Thailand
  • Berger, Antoine Department of Biology, University of Fribourg, Switzerland
  • Métraux, Jean-Pierre Department of Biology, University of Fribourg, Switzerland
  • L'Haridon, Floriane Department of Biology, University of Fribourg, Switzerland
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  • New Phytologist. - 2016, vol. 210, no. 3, p. 1033-1043
English We have explored the importance of the phyllosphere microbiome in plant resistance in the cuticle mutants bdg (BODYGUARD) or lacs2.3 (LONG CHAIN FATTY ACID SYNTHASE 2) that are strongly resistant to the fungal pathogen Botrytis cinerea. The study includes infection of plants under sterile conditions, 16S ribosomal DNA sequencing of the phyllosphere microbiome, and isolation and high coverage sequencing of bacteria from the phyllosphere. When inoculated under sterile conditions bdg became as susceptible as wild-type (WT) plants whereas lacs2.3 mutants retained the resistance. Adding washes of its phyllosphere microbiome could restore the resistance of bdg mutants, whereas the resistance of lacs2.3 results from endogenous mechanisms. The phyllosphere microbiome showed distinct populations in WT plants compared to cuticle mutants. One species identified as Pseudomonas sp isolated from the microbiome of bdg provided resistance to B. cinerea on Arabidopsis thaliana as well as on apple fruits. No direct activity was observed against B. cinerea and the action of the bacterium required the plant. Thus, microbes present on the plant surface contribute to the resistance to B. cinerea. These results open new perspectives on the function of the leaf microbiome in the protection of plants.
Faculté des sciences et de médecine
Département de Biologie
  • English
Biological sciences
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